Activation of rat thymocytes selectively upregulates the expression of somatostatin receptor subtype-1

M. Sedqi, Sabita Roy, D. Mohanraj, Sundaram Ramakrishnan, H. H. Loh

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Abstract

Somatostatin and other neuropeptides are known to modulate the proliferative capacity of immune cells. In the present study, we investigated the expression of Somatostatin receptor (SSTR) subtypes on rat thymocytes. RT-PCR analysis of fresh thymocytes showed significant levels of transcripts for the SSTR2 whereas transcripts for the SSTR1 and SSTR3 were not detectable. Interestingly, when the thymocytes were activated with low concentration of Phytohemagglutinin and interleukin 1, the transcript for SSTR1 was markedly increased. Lymphokine induced activation of thymocytes selectively upregulated the SSTR1 since, transcripts for SSTR2 remained the same after activation and SSTR3 was not detectable. PCR amplified fragment of SSTR1 from the activated thymocytes showed identical sequence to the rat brain receptor. The physiological significance of the increase of SSTR1 mRNA in thymocytes after activation remains to be elucidated but it may be possible that these two different subsets of receptors (SSTR1 and SSTR2) are involved in the modulation of thymocyte proliferation and differentiation.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume38
Issue number1 Included
StatePublished - Oct 22 1996
Externally publishedYes

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Somatostatin Receptors
Thymocytes
Rats
Up-Regulation
Chemical activation
Lymphokines
Polymerase Chain Reaction
Phytohemagglutinins
Somatostatin
Neuropeptides
Interleukin-1
somatostatin receptor type 1
Brain
Modulation
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Activation of rat thymocytes selectively upregulates the expression of somatostatin receptor subtype-1. / Sedqi, M.; Roy, Sabita; Mohanraj, D.; Ramakrishnan, Sundaram; Loh, H. H.

In: Biochemistry and Molecular Biology International, Vol. 38, No. 1 Included, 22.10.1996, p. 103-112.

Research output: Contribution to journalArticle

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