Abstract
Growth hormone-releasing hormone (GHRH) can act as a potent growth factor in various cancers. The mitogenic activity of this neuropeptide is exerted through binding to the pituitary type receptors (GHRH-R) or their splice variants (SV). In the present work, we studied whether this hormone can activate the JAK2/STAT3 pathway which plays a crucial role in cancer cell proliferation and is also linked to carcinogenesis. We transfected HeLa human cervical cancer cells, which are not sensitive to GHRH analogs with the pGHRH-R. Transfected cells responded to the GHRH or its antagonist with an increase or a decrease in proliferation, respectively. These results were confirmed by the expression of proliferating cell nuclear antigen. We then showed that these effects are linked to the activation of the JAK2/STAT3 pathway. Our work demonstrates the activation of JAK/STAT3 pathway by GHRH and sheds further light to the mechanisms of the antitumorogenic action of GHRH antagonists.
| Original language | English |
|---|---|
| Pages (from-to) | 959-964 |
| Number of pages | 6 |
| Journal | Cellular and Molecular Life Sciences |
| Volume | 67 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 1 2010 |
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Keywords
- GHRH
- Ghrh receptor
- JAK2/STAT3
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Molecular Medicine
- Pharmacology
- Cellular and Molecular Neuroscience
- Medicine(all)
Cite this
Activation of Janus kinase/signal transducer and activator of transcription 3 pathway by growth hormone-releasing hormone. / Siejka, Agnieszka; Schally, Andrew V; Barabutis, Nektarios.
In: Cellular and Molecular Life Sciences, Vol. 67, No. 6, 01.03.2010, p. 959-964.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of Janus kinase/signal transducer and activator of transcription 3 pathway by growth hormone-releasing hormone
AU - Siejka, Agnieszka
AU - Schally, Andrew V
AU - Barabutis, Nektarios
PY - 2010/3/1
Y1 - 2010/3/1
N2 - Growth hormone-releasing hormone (GHRH) can act as a potent growth factor in various cancers. The mitogenic activity of this neuropeptide is exerted through binding to the pituitary type receptors (GHRH-R) or their splice variants (SV). In the present work, we studied whether this hormone can activate the JAK2/STAT3 pathway which plays a crucial role in cancer cell proliferation and is also linked to carcinogenesis. We transfected HeLa human cervical cancer cells, which are not sensitive to GHRH analogs with the pGHRH-R. Transfected cells responded to the GHRH or its antagonist with an increase or a decrease in proliferation, respectively. These results were confirmed by the expression of proliferating cell nuclear antigen. We then showed that these effects are linked to the activation of the JAK2/STAT3 pathway. Our work demonstrates the activation of JAK/STAT3 pathway by GHRH and sheds further light to the mechanisms of the antitumorogenic action of GHRH antagonists.
AB - Growth hormone-releasing hormone (GHRH) can act as a potent growth factor in various cancers. The mitogenic activity of this neuropeptide is exerted through binding to the pituitary type receptors (GHRH-R) or their splice variants (SV). In the present work, we studied whether this hormone can activate the JAK2/STAT3 pathway which plays a crucial role in cancer cell proliferation and is also linked to carcinogenesis. We transfected HeLa human cervical cancer cells, which are not sensitive to GHRH analogs with the pGHRH-R. Transfected cells responded to the GHRH or its antagonist with an increase or a decrease in proliferation, respectively. These results were confirmed by the expression of proliferating cell nuclear antigen. We then showed that these effects are linked to the activation of the JAK2/STAT3 pathway. Our work demonstrates the activation of JAK/STAT3 pathway by GHRH and sheds further light to the mechanisms of the antitumorogenic action of GHRH antagonists.
KW - GHRH
KW - Ghrh receptor
KW - JAK2/STAT3
UR - http://www.scopus.com/inward/record.url?scp=77949500033&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949500033&partnerID=8YFLogxK
U2 - 10.1007/s00018-009-0224-y
DO - 10.1007/s00018-009-0224-y
M3 - Article
C2 - 20012909
AN - SCOPUS:77949500033
VL - 67
SP - 959
EP - 964
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
SN - 1420-682X
IS - 6
ER -