Activation of Janus kinase/signal transducer and activator of transcription 3 pathway by growth hormone-releasing hormone

Agnieszka Siejka, Andrew V. Schally, Nektarios Barabutis

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Growth hormone-releasing hormone (GHRH) can act as a potent growth factor in various cancers. The mitogenic activity of this neuropeptide is exerted through binding to the pituitary type receptors (GHRH-R) or their splice variants (SV). In the present work, we studied whether this hormone can activate the JAK2/STAT3 pathway which plays a crucial role in cancer cell proliferation and is also linked to carcinogenesis. We transfected HeLa human cervical cancer cells, which are not sensitive to GHRH analogs with the pGHRH-R. Transfected cells responded to the GHRH or its antagonist with an increase or a decrease in proliferation, respectively. These results were confirmed by the expression of proliferating cell nuclear antigen. We then showed that these effects are linked to the activation of the JAK2/STAT3 pathway. Our work demonstrates the activation of JAK/STAT3 pathway by GHRH and sheds further light to the mechanisms of the antitumorogenic action of GHRH antagonists.

Original languageEnglish (US)
Pages (from-to)959-964
Number of pages6
JournalCellular and Molecular Life Sciences
Volume67
Issue number6
DOIs
StatePublished - Mar 1 2010

Keywords

  • GHRH
  • Ghrh receptor
  • JAK2/STAT3

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Medicine(all)

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