Activation of human B lymphocytes. VII. The regulatory effect of cyclic adenosine monophosphate on human B cell activation

Paul Katz, Anthony S. Fauci

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cyclic adenosine monophosphate (cAMP) has been demonstrated to play an integral role in the regulation of B cell activation. By employing a plaque-forming cell (PFC) assay for polyclonal activation of human B lymphocytes, it was demonstrated that dibutyryl cyclic adenosine monophosphate (DB-cAMP) markedly increased the PFC response of pokeweed mitogen (PWM)-stimulated lymphocytes. Inducers of intracellular cAMP effected a comparable enhancement. Co-cultures of fresh lymphocytes with autologous T cells which had been pre-incubated with DB-cAMP produced an enhancement of B cell activation by a selective effect on the T cells. The mechanism of action of this enhancement of the B cell response is most likely a relative increase in helper T cell function resulting from a selective inhibition of suppressor T cells.

Original languageEnglish
Pages (from-to)334-338
Number of pages5
JournalThe Journal of Allergy and Clinical Immunology
Volume61
Issue number5
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

Fingerprint

Cyclic AMP
B-Lymphocytes
T-Lymphocytes
Lymphocytes
Pokeweed Mitogens
Helper-Inducer T-Lymphocytes
Coculture Techniques

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Activation of human B lymphocytes. VII. The regulatory effect of cyclic adenosine monophosphate on human B cell activation. / Katz, Paul; Fauci, Anthony S.

In: The Journal of Allergy and Clinical Immunology, Vol. 61, No. 5, 01.01.1978, p. 334-338.

Research output: Contribution to journalArticle

@article{f2820c753e4d4e8285c81c801f0c137b,
title = "Activation of human B lymphocytes. VII. The regulatory effect of cyclic adenosine monophosphate on human B cell activation",
abstract = "Cyclic adenosine monophosphate (cAMP) has been demonstrated to play an integral role in the regulation of B cell activation. By employing a plaque-forming cell (PFC) assay for polyclonal activation of human B lymphocytes, it was demonstrated that dibutyryl cyclic adenosine monophosphate (DB-cAMP) markedly increased the PFC response of pokeweed mitogen (PWM)-stimulated lymphocytes. Inducers of intracellular cAMP effected a comparable enhancement. Co-cultures of fresh lymphocytes with autologous T cells which had been pre-incubated with DB-cAMP produced an enhancement of B cell activation by a selective effect on the T cells. The mechanism of action of this enhancement of the B cell response is most likely a relative increase in helper T cell function resulting from a selective inhibition of suppressor T cells.",
author = "Paul Katz and Fauci, {Anthony S.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/0091-6749(78)90056-8",
language = "English",
volume = "61",
pages = "334--338",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Activation of human B lymphocytes. VII. The regulatory effect of cyclic adenosine monophosphate on human B cell activation

AU - Katz, Paul

AU - Fauci, Anthony S.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Cyclic adenosine monophosphate (cAMP) has been demonstrated to play an integral role in the regulation of B cell activation. By employing a plaque-forming cell (PFC) assay for polyclonal activation of human B lymphocytes, it was demonstrated that dibutyryl cyclic adenosine monophosphate (DB-cAMP) markedly increased the PFC response of pokeweed mitogen (PWM)-stimulated lymphocytes. Inducers of intracellular cAMP effected a comparable enhancement. Co-cultures of fresh lymphocytes with autologous T cells which had been pre-incubated with DB-cAMP produced an enhancement of B cell activation by a selective effect on the T cells. The mechanism of action of this enhancement of the B cell response is most likely a relative increase in helper T cell function resulting from a selective inhibition of suppressor T cells.

AB - Cyclic adenosine monophosphate (cAMP) has been demonstrated to play an integral role in the regulation of B cell activation. By employing a plaque-forming cell (PFC) assay for polyclonal activation of human B lymphocytes, it was demonstrated that dibutyryl cyclic adenosine monophosphate (DB-cAMP) markedly increased the PFC response of pokeweed mitogen (PWM)-stimulated lymphocytes. Inducers of intracellular cAMP effected a comparable enhancement. Co-cultures of fresh lymphocytes with autologous T cells which had been pre-incubated with DB-cAMP produced an enhancement of B cell activation by a selective effect on the T cells. The mechanism of action of this enhancement of the B cell response is most likely a relative increase in helper T cell function resulting from a selective inhibition of suppressor T cells.

UR - http://www.scopus.com/inward/record.url?scp=0018192012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018192012&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(78)90056-8

DO - 10.1016/0091-6749(78)90056-8

M3 - Article

VL - 61

SP - 334

EP - 338

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 5

ER -