Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p)

Carolyn M. Drazinic, Jeffrey B. Smerage, M. Cecilia López, Henry V. Baker

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Transcriptional activation in eukaryotic organisms normally requires combinatorial interactions of multiple transcription factors. In most cases, the precise rule played by each transcription factor is not known. The upstream activating sequence (UAS) elements of glycolytic enzyme genes in Saccharomyces cerevisiae are excellent model systems for the study of combinatorial interactions. The yeast protein known as Rap1p acts as both a transcriptional repressor and an activator, depending on sequence context. Rap1p-binding sites are found adjacent to Gcr1p-binding sites in the UAS elements of glycolytic enzyme genes. These UAS elements constitute some of the strongest activating sequences known in S. cerevisiae. In this study, we have investigated the relationship between Rap1p- and Gcr1p-binding sites and the proteins that bind them. In vivo DNA-binding studies with rap1(ts) mutant strains demonstrated that the inability of Rap1p to bind at its site resulted in the inability of Gcr1p to bind at adjacent binding sites. Synthetic oligonucleotides, modeled on the UAS element of PYK1, in which the relative positions of the Rap1p- and Gcr1p-binding sites were varied were prepared and tested for their ability to function as UAS elements. The ability of the oligonucleotides to function as UAS elements was dependent not only on the presence of both binding sites but also on the relative distance between the binding sites. In vivo DNA-binding studies showed that the ability of Rap1p to bind its site was independent of Gcr1p but that the ability of Gcr1p to bind its site was dependent on the presence of an appropriately spaced and bound Rap1p-binding site. In vitro binding studies showed Rap1p-enhanced binding of Gcr1p on oligonucleotides modeled after the native PYK1 UAS element but not when the Rap1p- and Gcr1p-binding sites were displaced by 5 nucleotides. This work demonstrates that the role of Rap1p in the activation of glycolytic enzyme genes is to bind in their UAS elements and to facilitate the binding of Gcr1p at adjacent binding sites.

Original languageEnglish (US)
Pages (from-to)3187-3196
Number of pages10
JournalMolecular and Cellular Biology
Volume16
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

Repressor Proteins
Transcription Factor AP-1
Transcription Factors
Binding Sites
Oligonucleotides
Saccharomyces cerevisiae
Genes
Enzyme Activation
Fungal Proteins
DNA
Enzymes
Transcriptional Activation
Carrier Proteins
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Drazinic, C. M., Smerage, J. B., Cecilia López, M., & Baker, H. V. (1996). Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p). Molecular and Cellular Biology, 16(6), 3187-3196.

Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p). / Drazinic, Carolyn M.; Smerage, Jeffrey B.; Cecilia López, M.; Baker, Henry V.

In: Molecular and Cellular Biology, Vol. 16, No. 6, 1996, p. 3187-3196.

Research output: Contribution to journalArticle

Drazinic, CM, Smerage, JB, Cecilia López, M & Baker, HV 1996, 'Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p)', Molecular and Cellular Biology, vol. 16, no. 6, pp. 3187-3196.
Drazinic, Carolyn M. ; Smerage, Jeffrey B. ; Cecilia López, M. ; Baker, Henry V. / Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p). In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 6. pp. 3187-3196.
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