Activated platelet aggregates in thrombotic thrombocytopenic purpura: Decrease with plasma infusions and normalization in remission

Yeon S. Ahn, Wenche Jy, Luciano Kolodny, Lawrence L. Horstman, Wei Wei Mao, Peter A. Valant, Robert C. Duncan

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Circulating activated platelet aggregates (aPA) were assayed by flow cytometry employing mAb α-CD62p in eight patients with thrombotic thrombocytopenic purpura (TTP). Elevation of aPA was observed in all patients in active stages of TTP; aPA normalized in remission. Plasma infusions with plasmapheresis decreased aPA in responding patients. The rise and fall of aPA preceded relapses and improvements, respectively. These changes were seen prior to the traditional indicators, LDH, haematocrit, and platelet count. Incubation of plasma from TTP patients with normal whole blood induced formation of aPA; this effect was significantly greater than that of plasmas from ITP patient controls (P < 0.01), suggesting the presence of an aPA-promoting factor in TTP plasma. Parallel experiments using a platelet aggregometer failed to detect effects of TTP plasma on normal blood. In summary, aPA appear to be a marker of disease activity, rising with relapse, falling with plasma therapy, and normalizing in remission. The now cytometric assay of aPA is more sensitive than aggregometry in detecting the putative aPA-promoting factor in TTP.

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalBritish Journal of Haematology
Volume95
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Flow cytometry
  • Plasma infusion
  • Platelet activation
  • Platelet aggregates
  • TTP

ASJC Scopus subject areas

  • Hematology

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