Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination

Cristina Fernandez-Valle, Douglas Gorman, Anna M. Gomez, Mary B Bunge

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Schwann cell (SC) differentiation into a myelinating cell requires concurrent interactions with basal lamina and an axon destined for myelination. As SCs differentiate, they undergo progressive morphological changes and initiate myelin-specific gene expression. We find that disrupting actin polymerization with cytochalasin D (CD) inhibits myelination of SC/neuron cocultures. Basal lamina is present, neurons are healthy, and the inhibition is reversible. Electron microscopic analysis reveals that actin plays a role at two stages of SC differentiation. At 0.75-1.0 μg/ml CD, SCs do not differentiate and appear as 'rounded' cells in contact with axons. This morphology is consistent with disruption of actin filaments and cell shape changes. However, at 0.25 μg/ml CD, SCs partially differentiate; they elongate and segregate axons but generally fail to form one-to-one relationships and spiral around the axon. In situ hybridizations reveal that SCs in CD-treated cultures do not express mRNAs encoding the myelin-specific proteins 2',3'-cyclic nucleotide phosphodiesterase (CNP), myelin-associated glycoprotein (MAG), and PO. Our results suggest that at the lower CD dose, SCs commence differentiation as evidenced by changes in cell shape but are unable to elaborate myelin lamellae because of a lack of myelin-specific mRNAs. We propose that F-actin influences myelin-specific gene expression in SCs.

Original languageEnglish
Pages (from-to)241-250
Number of pages10
JournalJournal of Neuroscience
Volume17
Issue number1
StatePublished - Jan 11 1997

Fingerprint

Cytochalasin D
Cell Shape
Schwann Cells
Actins
Myelin Sheath
Axons
Gene Expression
Basement Membrane
Cell Differentiation
2',3'-Cyclic-Nucleotide Phosphodiesterases
Myelin-Associated Glycoprotein
Myelin Proteins
Neurons
Messenger RNA
Coculture Techniques
Actin Cytoskeleton
Polymerization
In Situ Hybridization
Electrons

Keywords

  • actin
  • cytochalasin D
  • in situ hybridization
  • mRNA expression
  • myelination
  • Schwann cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fernandez-Valle, C., Gorman, D., Gomez, A. M., & Bunge, M. B. (1997). Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination. Journal of Neuroscience, 17(1), 241-250.

Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination. / Fernandez-Valle, Cristina; Gorman, Douglas; Gomez, Anna M.; Bunge, Mary B.

In: Journal of Neuroscience, Vol. 17, No. 1, 11.01.1997, p. 241-250.

Research output: Contribution to journalArticle

Fernandez-Valle, C, Gorman, D, Gomez, AM & Bunge, MB 1997, 'Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination', Journal of Neuroscience, vol. 17, no. 1, pp. 241-250.
Fernandez-Valle, Cristina ; Gorman, Douglas ; Gomez, Anna M. ; Bunge, Mary B. / Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 1. pp. 241-250.
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