Actin-associated cell-surface glycoprotein from ascites cell microvilli: A disulfide-linked multimer

G. Jung, David Andrews, K. L. Carraway, C. A. Carothers Carraway

Research output: Contribution to journalArticle

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Abstract

Isolated microvilli of the MAT-C1 subline of the 13762 rat mammary adenocarcinoma contain a transmembrane complex composed of a cell surface, cytoskeleton-associated glycoprotein (CAG), actin, and a 58,000-dalton polypeptide (58K). The behavior of CAG has been studied by differential centrifugation and velocity sedimentation gradient centrifugation of detergent extracts of microvilli. CAG can be pelleted along with a fraction of the microvillar actin even in the presence of ionic detergents and under microfilament-depolymerizing conditions. By velocity sedimentation analysis CAG in Triton/PBS extracts sediments as a large, heterogeneous species (sedimentation coefficient > 25S). In Sarkosyl and sodium dodecyl sulfate (SDS) the size and heterogeneity are somewhat reduced. In SDS CAG sediments as a 20S species in the absence of mercaptoethanol and as a 5S species in the presence of mercaptoethanol. These results indicate that CAG is a disulfide-linked multimer in the microvillus membrane. We suggest that the stable multimeric structure of CAG permits it to act as the membrane association site for several microfilaments and plays an important role in the formation and stabilization of the microvillus structure.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalJournal of Cellular Biochemistry
Volume28
Issue number4
StatePublished - Dec 1 1985

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Membrane Glycoproteins
Microvilli
Cytoskeleton
Ascites
Disulfides
Actins
Glycoproteins
Sedimentation
Centrifugation
Mercaptoethanol
Actin Cytoskeleton
Sodium Dodecyl Sulfate
Detergents
Sediments
Membranes
Rats
Adenocarcinoma
Breast
Stabilization
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Actin-associated cell-surface glycoprotein from ascites cell microvilli : A disulfide-linked multimer. / Jung, G.; Andrews, David; Carraway, K. L.; Carothers Carraway, C. A.

In: Journal of Cellular Biochemistry, Vol. 28, No. 4, 01.12.1985, p. 243-252.

Research output: Contribution to journalArticle

Jung, G. ; Andrews, David ; Carraway, K. L. ; Carothers Carraway, C. A. / Actin-associated cell-surface glycoprotein from ascites cell microvilli : A disulfide-linked multimer. In: Journal of Cellular Biochemistry. 1985 ; Vol. 28, No. 4. pp. 243-252.
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