Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis

Tae Won Hahn, Terrence O'Brien, Woo Jin Sah, Jae Ho Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acridine orange staining from corneal scrapings and contact lens solutions for the rapid diagnosis of four consecutive cases of Acanthamoeba keratitis. Gram stain and culture on nonnutrient agar plates with Escherichia coli overlay were also made. Corneal scrapings stained with acridine orange revealed yellow-to-orange polygonal, cystic structures consistent with the appearance of Acanthamoeba among inflammatory cells and the corneal epithelial cells. The contact lens case solutions of two patients also showed numerous cysts with double wall. Some organisms from the third patient were identified as Acanthamoeba castellani and others as Acanthamoeba lugdunensis. Based on the acridine orange staining results in four cases of Acanthamoeba keratitis, this stain is recommended as a simple and reliable method for the rapid diagnosis of this disease.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalJapanese Journal of Ophthalmology
Volume42
Issue number2
DOIs
StatePublished - Mar 1 1998
Externally publishedYes

Fingerprint

Acanthamoeba Keratitis
Acridine Orange
Acanthamoeba
Contact Lens Solutions
Staining and Labeling
Herpetic Keratitis
Delayed Diagnosis
Diagnostic Errors
Cornea
Agar
Communicable Diseases
Cysts
Coloring Agents
Epithelial Cells
Escherichia coli
Inflammation

Keywords

  • Acanthamoeba keratitis
  • Acridine orange stain
  • Contact lens

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis. / Hahn, Tae Won; O'Brien, Terrence; Sah, Woo Jin; Kim, Jae Ho.

In: Japanese Journal of Ophthalmology, Vol. 42, No. 2, 01.03.1998, p. 108-114.

Research output: Contribution to journalArticle

Hahn, Tae Won ; O'Brien, Terrence ; Sah, Woo Jin ; Kim, Jae Ho. / Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis. In: Japanese Journal of Ophthalmology. 1998 ; Vol. 42, No. 2. pp. 108-114.
@article{ffff8a1ad6d24ef58bcfa6cc6f59b60f,
title = "Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis",
abstract = "Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acridine orange staining from corneal scrapings and contact lens solutions for the rapid diagnosis of four consecutive cases of Acanthamoeba keratitis. Gram stain and culture on nonnutrient agar plates with Escherichia coli overlay were also made. Corneal scrapings stained with acridine orange revealed yellow-to-orange polygonal, cystic structures consistent with the appearance of Acanthamoeba among inflammatory cells and the corneal epithelial cells. The contact lens case solutions of two patients also showed numerous cysts with double wall. Some organisms from the third patient were identified as Acanthamoeba castellani and others as Acanthamoeba lugdunensis. Based on the acridine orange staining results in four cases of Acanthamoeba keratitis, this stain is recommended as a simple and reliable method for the rapid diagnosis of this disease.",
keywords = "Acanthamoeba keratitis, Acridine orange stain, Contact lens",
author = "Hahn, {Tae Won} and Terrence O'Brien and Sah, {Woo Jin} and Kim, {Jae Ho}",
year = "1998",
month = "3",
day = "1",
doi = "10.1016/S0021-5155(97)00127-5",
language = "English",
volume = "42",
pages = "108--114",
journal = "Japanese Journal of Ophthalmology",
issn = "0021-5155",
publisher = "Springer Japan",
number = "2",

}

TY - JOUR

T1 - Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis

AU - Hahn, Tae Won

AU - O'Brien, Terrence

AU - Sah, Woo Jin

AU - Kim, Jae Ho

PY - 1998/3/1

Y1 - 1998/3/1

N2 - Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acridine orange staining from corneal scrapings and contact lens solutions for the rapid diagnosis of four consecutive cases of Acanthamoeba keratitis. Gram stain and culture on nonnutrient agar plates with Escherichia coli overlay were also made. Corneal scrapings stained with acridine orange revealed yellow-to-orange polygonal, cystic structures consistent with the appearance of Acanthamoeba among inflammatory cells and the corneal epithelial cells. The contact lens case solutions of two patients also showed numerous cysts with double wall. Some organisms from the third patient were identified as Acanthamoeba castellani and others as Acanthamoeba lugdunensis. Based on the acridine orange staining results in four cases of Acanthamoeba keratitis, this stain is recommended as a simple and reliable method for the rapid diagnosis of this disease.

AB - Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acridine orange staining from corneal scrapings and contact lens solutions for the rapid diagnosis of four consecutive cases of Acanthamoeba keratitis. Gram stain and culture on nonnutrient agar plates with Escherichia coli overlay were also made. Corneal scrapings stained with acridine orange revealed yellow-to-orange polygonal, cystic structures consistent with the appearance of Acanthamoeba among inflammatory cells and the corneal epithelial cells. The contact lens case solutions of two patients also showed numerous cysts with double wall. Some organisms from the third patient were identified as Acanthamoeba castellani and others as Acanthamoeba lugdunensis. Based on the acridine orange staining results in four cases of Acanthamoeba keratitis, this stain is recommended as a simple and reliable method for the rapid diagnosis of this disease.

KW - Acanthamoeba keratitis

KW - Acridine orange stain

KW - Contact lens

UR - http://www.scopus.com/inward/record.url?scp=0032029283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032029283&partnerID=8YFLogxK

U2 - 10.1016/S0021-5155(97)00127-5

DO - 10.1016/S0021-5155(97)00127-5

M3 - Article

VL - 42

SP - 108

EP - 114

JO - Japanese Journal of Ophthalmology

JF - Japanese Journal of Ophthalmology

SN - 0021-5155

IS - 2

ER -