Acidocalcisomes of Phytomonas françai possess distinct morphological characteristics and contain iron

Kildare Miranda, Claudia D Rodrigues, Joachim Hentchel, Anibal Vercesi, Helmut Plattner, Wanderley De Souza, Roberto Docampo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Acidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites, and recently found in other unicellular eukaryotes. The aim of this study was to identify the presence of acidocalcisomes in the plant trypanosomatid Phytomonas françai. Electron-dense organelles of P. françai were shown to contain large amounts of oxygen, sodium, magnesium, phosphorus, potassium, calcium, iron, and zinc as determined by X-ray microanalysis, either in situ or when purified using iodixanol gradient centrifugation or by elemental mapping. The presence of iron is not common in other acidocalcisomes. In situ, but not when purified, these organelles showed an elongated shape differing from previously described acidocalcisomes. However, these organelles also possessed a vacuolar H +-pyrophosphatase (V-H+-PPase) as determined by biochemical methods and by immunofluorescence microscopy using antibodies against the enzyme. Together, these results suggest that the electron-dense organelles of P. françai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content.

Original languageEnglish
Pages (from-to)647-655
Number of pages9
JournalMicroscopy and Microanalysis
Volume10
Issue number5
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

organelles
Calcium
Iron
iron
Centrifugation
Electrons
Microanalysis
Antibodies
Magnesium
Potassium
Phosphorus
calcium
Microscopic examination
Zinc
Enzymes
Sodium
eukaryotes
parasites
X rays
Oxygen

Keywords

  • Acidocalcisomes
  • Calcium
  • Elemental mapping
  • Phytomonas
  • Pyrophosphatase
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Acidocalcisomes of Phytomonas françai possess distinct morphological characteristics and contain iron. / Miranda, Kildare; Rodrigues, Claudia D; Hentchel, Joachim; Vercesi, Anibal; Plattner, Helmut; De Souza, Wanderley; Docampo, Roberto.

In: Microscopy and Microanalysis, Vol. 10, No. 5, 01.10.2004, p. 647-655.

Research output: Contribution to journalArticle

Miranda, Kildare ; Rodrigues, Claudia D ; Hentchel, Joachim ; Vercesi, Anibal ; Plattner, Helmut ; De Souza, Wanderley ; Docampo, Roberto. / Acidocalcisomes of Phytomonas françai possess distinct morphological characteristics and contain iron. In: Microscopy and Microanalysis. 2004 ; Vol. 10, No. 5. pp. 647-655.
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