Abstract
The acid-sensitive cation channel acid-sensing ion channel-2 (ASIC2) is widely believed to be a receptor for acid (sour) taste in mammals on the basis of its physiological properties and expression in rat taste bud cells. Using reverse transcriptase-PCR, we detected expression of ASIC1 and ASIC3, but not ASIC4, in mouse and rat taste buds and nonsensory lingual epithelium. Surprisingly, we did not detect mRNA for ASIC2 in mouse taste buds, although we readily observed its expression in rat taste buds. Furthermore, in Ca 2+ imaging experiments, ASIC2 knock-out mice exhibited normal physiological responses (increases in intracellular Ca2+ concentrations) to acid taste stimuli. Our results indicate that ASIC2 is not required for acid taste in mice, and that if a universal mammalian acid taste transduction mechanism exists, it likely uses other acid-sensitive receptors or ion channels.
| Original language | English |
|---|---|
| Pages (from-to) | 4088-4091 |
| Number of pages | 4 |
| Journal | Journal of Neuroscience |
| Volume | 24 |
| Issue number | 16 |
| DOIs | |
| State | Published - Apr 21 2004 |
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Keywords
- Acid
- ASIC
- Receptor
- Sour
- Taste
- Transduction
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Acid-Sensing Ion Channel-2 Is Not Necessary for Sour Taste in Mice. / Richter, Trevor A.; Dvoryanchikov, Gennady A.; Roper, Stephen D; Chaudhari, Nirupa.
In: Journal of Neuroscience, Vol. 24, No. 16, 21.04.2004, p. 4088-4091.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acid-Sensing Ion Channel-2 Is Not Necessary for Sour Taste in Mice
AU - Richter, Trevor A.
AU - Dvoryanchikov, Gennady A.
AU - Roper, Stephen D
AU - Chaudhari, Nirupa
PY - 2004/4/21
Y1 - 2004/4/21
N2 - The acid-sensitive cation channel acid-sensing ion channel-2 (ASIC2) is widely believed to be a receptor for acid (sour) taste in mammals on the basis of its physiological properties and expression in rat taste bud cells. Using reverse transcriptase-PCR, we detected expression of ASIC1 and ASIC3, but not ASIC4, in mouse and rat taste buds and nonsensory lingual epithelium. Surprisingly, we did not detect mRNA for ASIC2 in mouse taste buds, although we readily observed its expression in rat taste buds. Furthermore, in Ca 2+ imaging experiments, ASIC2 knock-out mice exhibited normal physiological responses (increases in intracellular Ca2+ concentrations) to acid taste stimuli. Our results indicate that ASIC2 is not required for acid taste in mice, and that if a universal mammalian acid taste transduction mechanism exists, it likely uses other acid-sensitive receptors or ion channels.
AB - The acid-sensitive cation channel acid-sensing ion channel-2 (ASIC2) is widely believed to be a receptor for acid (sour) taste in mammals on the basis of its physiological properties and expression in rat taste bud cells. Using reverse transcriptase-PCR, we detected expression of ASIC1 and ASIC3, but not ASIC4, in mouse and rat taste buds and nonsensory lingual epithelium. Surprisingly, we did not detect mRNA for ASIC2 in mouse taste buds, although we readily observed its expression in rat taste buds. Furthermore, in Ca 2+ imaging experiments, ASIC2 knock-out mice exhibited normal physiological responses (increases in intracellular Ca2+ concentrations) to acid taste stimuli. Our results indicate that ASIC2 is not required for acid taste in mice, and that if a universal mammalian acid taste transduction mechanism exists, it likely uses other acid-sensitive receptors or ion channels.
KW - Acid
KW - ASIC
KW - Receptor
KW - Sour
KW - Taste
KW - Transduction
UR - http://www.scopus.com/inward/record.url?scp=2142707226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2142707226&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.0653-04.2004
DO - 10.1523/JNEUROSCI.0653-04.2004
M3 - Article
C2 - 15102924
AN - SCOPUS:2142707226
VL - 24
SP - 4088
EP - 4091
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 16
ER -