Acid-base, plasma ion and blood gas changes in rainbow trout during short term toxic zinc exposure

Douglas J. Spry, Chris M. Wood

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

1. Rainbow trout exposed to waterborne zinc at an acutely lethal level (1.5 mg/l) or at a lower concentration close to the 4 d LC50 (0.8 mg/l) exhibited contrasting physiological responses in artificial soft water (ASW). 2. The changes in acid-base status and other blood parameters during the acute zinc exposure (1.5 mg/l) in ASW resulted from a rapid cascade of events in which hypoxemia, probably due to gill damage, resulted in tissue hypoxia and a mixed acidosis which were rapidly fatal. Changes in Het, MCHC, Cl- and lactate reflected the acidosis. Hypoxia rather than acidosis was the primary lethal mechanism. 3. Lower level zinc exposure (0.8 mg/l) over a 3 day period resulted in a slight alkalosis, despite a rise in {Mathematical expression}. No changes were observed in plasma concentrations of Na+, Cl- or K+, and {Mathematical expression} remained high. Possible causes for the alkalosis are discussed. Some mortality occurred, suggesting that toxic mechanisms other than hypoxemia may have operated. 4. Zinc accumulated in whole blood to a greater extent during the 3 day low level exposure than the short term, high level exposure, suggesting that toxicity in the latter reflected an external effect of zinc. Zinc accumulated only in plasma with no penetration of the RBC's.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalJournal of Comparative Physiology B
Volume154
Issue number2
DOIs
StatePublished - Mar 1 1984

Fingerprint

Poisons
Oncorhynchus mykiss
blood gases
rainbow
Zinc
Blood
Gases
blood
zinc
Ions
ions
Plasmas
plasma
Acids
ion
hypoxia
acids
acid
acidosis
Acidosis

ASJC Scopus subject areas

  • Physiology (medical)
  • Environmental Science(all)
  • Physiology
  • Animal Science and Zoology

Cite this

Acid-base, plasma ion and blood gas changes in rainbow trout during short term toxic zinc exposure. / Spry, Douglas J.; Wood, Chris M.

In: Journal of Comparative Physiology B, Vol. 154, No. 2, 01.03.1984, p. 149-158.

Research output: Contribution to journalArticle

@article{e10cbc7ca5024836890f897db32867fa,
title = "Acid-base, plasma ion and blood gas changes in rainbow trout during short term toxic zinc exposure",
abstract = "1. Rainbow trout exposed to waterborne zinc at an acutely lethal level (1.5 mg/l) or at a lower concentration close to the 4 d LC50 (0.8 mg/l) exhibited contrasting physiological responses in artificial soft water (ASW). 2. The changes in acid-base status and other blood parameters during the acute zinc exposure (1.5 mg/l) in ASW resulted from a rapid cascade of events in which hypoxemia, probably due to gill damage, resulted in tissue hypoxia and a mixed acidosis which were rapidly fatal. Changes in Het, MCHC, Cl- and lactate reflected the acidosis. Hypoxia rather than acidosis was the primary lethal mechanism. 3. Lower level zinc exposure (0.8 mg/l) over a 3 day period resulted in a slight alkalosis, despite a rise in {Mathematical expression}. No changes were observed in plasma concentrations of Na+, Cl- or K+, and {Mathematical expression} remained high. Possible causes for the alkalosis are discussed. Some mortality occurred, suggesting that toxic mechanisms other than hypoxemia may have operated. 4. Zinc accumulated in whole blood to a greater extent during the 3 day low level exposure than the short term, high level exposure, suggesting that toxicity in the latter reflected an external effect of zinc. Zinc accumulated only in plasma with no penetration of the RBC's.",
author = "Spry, {Douglas J.} and Wood, {Chris M.}",
year = "1984",
month = "3",
day = "1",
doi = "10.1007/BF00684139",
language = "English",
volume = "154",
pages = "149--158",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
issn = "0174-1578",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Acid-base, plasma ion and blood gas changes in rainbow trout during short term toxic zinc exposure

AU - Spry, Douglas J.

AU - Wood, Chris M.

PY - 1984/3/1

Y1 - 1984/3/1

N2 - 1. Rainbow trout exposed to waterborne zinc at an acutely lethal level (1.5 mg/l) or at a lower concentration close to the 4 d LC50 (0.8 mg/l) exhibited contrasting physiological responses in artificial soft water (ASW). 2. The changes in acid-base status and other blood parameters during the acute zinc exposure (1.5 mg/l) in ASW resulted from a rapid cascade of events in which hypoxemia, probably due to gill damage, resulted in tissue hypoxia and a mixed acidosis which were rapidly fatal. Changes in Het, MCHC, Cl- and lactate reflected the acidosis. Hypoxia rather than acidosis was the primary lethal mechanism. 3. Lower level zinc exposure (0.8 mg/l) over a 3 day period resulted in a slight alkalosis, despite a rise in {Mathematical expression}. No changes were observed in plasma concentrations of Na+, Cl- or K+, and {Mathematical expression} remained high. Possible causes for the alkalosis are discussed. Some mortality occurred, suggesting that toxic mechanisms other than hypoxemia may have operated. 4. Zinc accumulated in whole blood to a greater extent during the 3 day low level exposure than the short term, high level exposure, suggesting that toxicity in the latter reflected an external effect of zinc. Zinc accumulated only in plasma with no penetration of the RBC's.

AB - 1. Rainbow trout exposed to waterborne zinc at an acutely lethal level (1.5 mg/l) or at a lower concentration close to the 4 d LC50 (0.8 mg/l) exhibited contrasting physiological responses in artificial soft water (ASW). 2. The changes in acid-base status and other blood parameters during the acute zinc exposure (1.5 mg/l) in ASW resulted from a rapid cascade of events in which hypoxemia, probably due to gill damage, resulted in tissue hypoxia and a mixed acidosis which were rapidly fatal. Changes in Het, MCHC, Cl- and lactate reflected the acidosis. Hypoxia rather than acidosis was the primary lethal mechanism. 3. Lower level zinc exposure (0.8 mg/l) over a 3 day period resulted in a slight alkalosis, despite a rise in {Mathematical expression}. No changes were observed in plasma concentrations of Na+, Cl- or K+, and {Mathematical expression} remained high. Possible causes for the alkalosis are discussed. Some mortality occurred, suggesting that toxic mechanisms other than hypoxemia may have operated. 4. Zinc accumulated in whole blood to a greater extent during the 3 day low level exposure than the short term, high level exposure, suggesting that toxicity in the latter reflected an external effect of zinc. Zinc accumulated only in plasma with no penetration of the RBC's.

UR - http://www.scopus.com/inward/record.url?scp=0021327182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021327182&partnerID=8YFLogxK

U2 - 10.1007/BF00684139

DO - 10.1007/BF00684139

M3 - Article

VL - 154

SP - 149

EP - 158

JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

SN - 0174-1578

IS - 2

ER -