Acetylcholine is released from taste cells, enhancing taste signalling

Robin Dando, Stephen D Roper

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Acetylcholine (ACh), a candidate neurotransmitter that has been implicated in taste buds, elicits calcium mobilization in Receptor (Type II) taste cells. Using RT-PCR analysis and pharmacological interventions, we demonstrate that the muscarinic acetylcholine receptor M3 mediates these actions. Applying ACh enhanced both taste-evoked Ca2+ responses and taste-evoked afferent neurotransmitter (ATP) secretion from taste Receptor cells. Blocking muscarinic receptors depressed taste-evoked responses in Receptor cells, suggesting that ACh is normally released from taste cells during taste stimulation. ACh biosensors confirmed that, indeed, taste Receptor cells secrete acetylcholine during gustatory stimulation. Genetic deletion of muscarinic receptors resulted in significantly diminished ATP secretion from taste buds. The data demonstrate a new role for acetylcholine as a taste bud transmitter. Our results imply specifically that ACh is an autocrine transmitter secreted by taste Receptor cells during gustatory stimulation, enhancing taste-evoked responses and afferent transmitter secretion.

Original languageEnglish
Pages (from-to)3009-3017
Number of pages9
JournalJournal of Physiology
Volume590
Issue number13
DOIs
StatePublished - Jul 1 2012

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Acetylcholine
Taste Buds
Muscarinic Receptors
Neurotransmitter Agents
Adenosine Triphosphate
Muscarinic M3 Receptors
Biosensing Techniques
Pharmacology
Calcium
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Physiology

Cite this

Acetylcholine is released from taste cells, enhancing taste signalling. / Dando, Robin; Roper, Stephen D.

In: Journal of Physiology, Vol. 590, No. 13, 01.07.2012, p. 3009-3017.

Research output: Contribution to journalArticle

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