Acellular collagen matrix as a possible 'off the shelf' biomaterial for urethral repair

Fang Chen, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

281 Citations (Scopus)

Abstract

Objectives. To determine whether a naturally derived collagen-based tissue substitute developed for urethral reconstruction would be suitable for urethral repair in an animal model. Several urethral conditions often require nongenital tissues for reconstruction, such as skin grafts or mucosal grafts from the bladder or buccal regions. However, the use of these tissues for urethroplasty may be associated with additional procedures for graft retrieval, prolonged hospitalization, and morbidity. Methods. A ventral urethral defect was created in 10 male rabbits. The acellular collagen matrix, obtained and processed from porcine bladder submucosa, was trimmed and used to replace the urethral defect in an onlay fashion. Serial urethrography was performed pre- and postoperatively at 0.5, 1, 2, 3, and 6 months. The animals were sacrificed 0.5, 1, 2, 3, and 6 months after surgery. The retrieved implants were analyzed grossly, histologically, and with immunocytochemistry. Results. All animals survived until being sacrificed without any noticeable voiding dysfunction. Serial urethrograms confirmed the maintenance of a wide urethral caliber without any signs of strictures. Gross examination at retrieval showed normally appearing tissue without any evidence of fibrosis. Histologically, the implanted matrices contained host cell infiltration and generous anglogenesis by 2 weeks after surgery. The presence of a confluent transitional cell layer was confirmed by immunocytochemical analyses using pancytokeratin antibodies. Anti-alpha actin antibodies demonstrated the migration of unorganized muscle fiber bundles 2 months after implantation and organized muscle bundles 6 months after implantation. Conclusions. The acellular collagen matrix appears to be a useful material for urethral repair in the rabbit. The matrix can be processed easily, has good characteristics for tissue handling and urethral function, and has the advantage of being an 'off the shelf' material.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalUrology
Volume54
Issue number3
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

Fingerprint

Biocompatible Materials
Collagen
Transplants
Urinary Bladder
Rabbits
Muscles
Inlays
Antibodies
Cheek
Actins
Pathologic Constriction
Hospitalization
Fibrosis
Swine
Animal Models
Immunohistochemistry
Maintenance
Morbidity
Skin

ASJC Scopus subject areas

  • Urology

Cite this

Acellular collagen matrix as a possible 'off the shelf' biomaterial for urethral repair. / Chen, Fang; Yoo, James J.; Atala, Anthony.

In: Urology, Vol. 54, No. 3, 01.09.1999, p. 407-410.

Research output: Contribution to journalArticle

Chen, Fang ; Yoo, James J. ; Atala, Anthony. / Acellular collagen matrix as a possible 'off the shelf' biomaterial for urethral repair. In: Urology. 1999 ; Vol. 54, No. 3. pp. 407-410.
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