Acceleration of chronic failure of intrahepatic canine islet autografts by a short course of prednisone

H. L R Rilo, P. B. Carroll, Y. J. Zeng, P. Fontes, J. Demetris, Camillo Ricordi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A topic of current interest in islet transplantation is the selection of an optimal site for long-term graft survival since the intrahepatic site may be characterized by long-term failure. Additionally, the use of immunosuppressive agents such as prednisone may adversely affect long-term graft function. In this study, we examined the long-term outcome of intrahepatic canine islet autografts and compared this with results obtained in animals treated with a short-term course of steroids or steroids plus insulin. Islets were isolated using the automated method and were purified on discontinuous EuroCollins Ficoll gradients (densities: 1.108, 1.096, 1.037). Prednisone-treated dogs were hyperglycemic during treatment but returned to normoglycemia after steroid withdrawal. Control and insulin-treated animals were normoglycemic following autotransplant, with no difference in plasma glucose levels between controls and the insulin-treated animals. All control dogs became diabetic at 11, 14, 17, and 19 months following islet autograft. Prednisone-treated dogs had more rapid onset of diabetes at 7, 11, and 12 months following ITx. Prednisone-treated dogs given insulin became hyperglycemic at 10, 14, 18, and 19 months post ITx. Graft failure was preceded by a decline in IVGTT K(g) values and diminished insulin secretion. At the time of graft failure islets showed no lymphocytic infiltration and islets stained positive for glucagon but few insulin-containing cells were seen. Thus, even when an initially adequate B cell mass was transplanted, the intrahepatic site was characterized by long-term canine autograft failure. A short course of prednisone accelerated the time to graft failure and insulin treatment reversed this acceleration.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalTransplantation
Volume57
Issue number2
StatePublished - Jan 1 1994
Externally publishedYes

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Autografts
Prednisone
Canidae
Insulin
Dogs
Transplants
Steroids
Islets of Langerhans Transplantation
Ficoll
Graft Survival
Immunosuppressive Agents
Glucagon
Treatment Failure
Curriculum
B-Lymphocytes
Glucose

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Rilo, H. L. R., Carroll, P. B., Zeng, Y. J., Fontes, P., Demetris, J., & Ricordi, C. (1994). Acceleration of chronic failure of intrahepatic canine islet autografts by a short course of prednisone. Transplantation, 57(2), 181-187.

Acceleration of chronic failure of intrahepatic canine islet autografts by a short course of prednisone. / Rilo, H. L R; Carroll, P. B.; Zeng, Y. J.; Fontes, P.; Demetris, J.; Ricordi, Camillo.

In: Transplantation, Vol. 57, No. 2, 01.01.1994, p. 181-187.

Research output: Contribution to journalArticle

Rilo, HLR, Carroll, PB, Zeng, YJ, Fontes, P, Demetris, J & Ricordi, C 1994, 'Acceleration of chronic failure of intrahepatic canine islet autografts by a short course of prednisone', Transplantation, vol. 57, no. 2, pp. 181-187.
Rilo, H. L R ; Carroll, P. B. ; Zeng, Y. J. ; Fontes, P. ; Demetris, J. ; Ricordi, Camillo. / Acceleration of chronic failure of intrahepatic canine islet autografts by a short course of prednisone. In: Transplantation. 1994 ; Vol. 57, No. 2. pp. 181-187.
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