Absence of mitochondrial dysfunction in polymyalgia rheumatica

Oscar Miró, Diana Jarreta, Jordi Casademont, Antoni Barrientos, Benjamín Rodríguez, Montserrat Gómez, Virginia Nunes, Álvaro Urbano-Márquez, Francesc Cardellach

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objective: To investigate the molecular and biochemical profile of skeletal muscle mitochondria of patients with isolated polymyalgia rheumatica (PMR). Patients and Methods: We included patients with a recent diagnosis of PMR and as control healthy individuals submitted to orthopedic surgery. Skeletal muscle was obtained from quadriceps, thus was mitochondria immediately isolated. Long polymerase chain reaction and Southern blot transference were performed to detect deleted mtDNA molecules. Mitochondrial oxidative activity using different substrates and individual enzyme activity of respiratory chain complexes were assessed to search for any biochemical dysfunction. Results: Fifty-one individuals (PMR = 25, controls = 26) were included. Mean age was 72 (11) years; 45% were females. We found no significant increase of deleted mtDNA molecules in PMR patients compared to controls. Both groups differed neither on oxygen consumption (p = NS for all substrates) nor enzymatic activity (p = NS for all complexes). Conclusions: Skeletal muscle mitochondria are molecularly and biochemically unaffected in PMR.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalScandinavian Journal of Rheumatology
Volume28
Issue number5
DOIs
StatePublished - Oct 29 1999
Externally publishedYes

Keywords

  • Electron transport chain
  • Enzyme activity
  • Mitochondria
  • Mitochondrial DNA
  • Mitochondrial DNA deletions
  • Oxidative activity
  • Polymyalgia rheumatica

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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  • Cite this

    Miró, O., Jarreta, D., Casademont, J., Barrientos, A., Rodríguez, B., Gómez, M., Nunes, V., Urbano-Márquez, Á., & Cardellach, F. (1999). Absence of mitochondrial dysfunction in polymyalgia rheumatica. Scandinavian Journal of Rheumatology, 28(5), 319-323. https://doi.org/10.1080/03009749950155526