Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans

Thierry Berney, Ruth Molano, Antonello Pileggi, Pierre Cattan, Caterina Vizzardelli, Camillo Ricordi, Luca A Inverardi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p= 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.

Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalCell Transplantation
Volume10
Issue number7
StatePublished - Dec 1 2001

Fingerprint

Macrophage Colony-Stimulating Factor
Macrophages
Islets of Langerhans
Transplants
Medical problems
Interleukin-1
Grafts
Tissue
Interleukin-6
Cytokines

Keywords

  • Islet primary nonfunction
  • Islet transplantation
  • Macrophages
  • Osteopetrotic mice

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Berney, T., Molano, R., Pileggi, A., Cattan, P., Vizzardelli, C., Ricordi, C., & Inverardi, L. A. (2001). Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. Cell Transplantation, 10(7), 633-637.

Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. / Berney, Thierry; Molano, Ruth; Pileggi, Antonello; Cattan, Pierre; Vizzardelli, Caterina; Ricordi, Camillo; Inverardi, Luca A.

In: Cell Transplantation, Vol. 10, No. 7, 01.12.2001, p. 633-637.

Research output: Contribution to journalArticle

Berney, T, Molano, R, Pileggi, A, Cattan, P, Vizzardelli, C, Ricordi, C & Inverardi, LA 2001, 'Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans', Cell Transplantation, vol. 10, no. 7, pp. 633-637.
Berney T, Molano R, Pileggi A, Cattan P, Vizzardelli C, Ricordi C et al. Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. Cell Transplantation. 2001 Dec 1;10(7):633-637.
Berney, Thierry ; Molano, Ruth ; Pileggi, Antonello ; Cattan, Pierre ; Vizzardelli, Caterina ; Ricordi, Camillo ; Inverardi, Luca A. / Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. In: Cell Transplantation. 2001 ; Vol. 10, No. 7. pp. 633-637.
@article{b6bef2dc26574f499b1ae8c9d08b6146,
title = "Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans",
abstract = "A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p= 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.",
keywords = "Islet primary nonfunction, Islet transplantation, Macrophages, Osteopetrotic mice",
author = "Thierry Berney and Ruth Molano and Antonello Pileggi and Pierre Cattan and Caterina Vizzardelli and Camillo Ricordi and Inverardi, {Luca A}",
year = "2001",
month = "12",
day = "1",
language = "English",
volume = "10",
pages = "633--637",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "7",

}

TY - JOUR

T1 - Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans

AU - Berney, Thierry

AU - Molano, Ruth

AU - Pileggi, Antonello

AU - Cattan, Pierre

AU - Vizzardelli, Caterina

AU - Ricordi, Camillo

AU - Inverardi, Luca A

PY - 2001/12/1

Y1 - 2001/12/1

N2 - A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p= 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.

AB - A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p= 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.

KW - Islet primary nonfunction

KW - Islet transplantation

KW - Macrophages

KW - Osteopetrotic mice

UR - http://www.scopus.com/inward/record.url?scp=0035573026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035573026&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 633

EP - 637

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 7

ER -