Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans

Thierry Berney, R. Damaris Molano, Antonello Pileggi, Pierre Cattan, Caterina Vizzardelli, Camillo Ricordi, Luca Inverardi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A role of macrophage-mediated inflammatory events in early islet graft loss is increasingly acknowledged. Osteopetrotic mice (op/op) have a complete absence of CSF-1, and thus of most tissue macrophages. We have investigated whether the absence of CSF-1-dependent macrophages in the graft itself or at the transplant site could decrease the delay to function of a syngeneic marginal islet mass. Islets transplanted into op/op or control recipients reversed diabetes in 59 days vs. 10 days (p= 0.28, NS). Islets isolated from op/op or control mice reversed diabetes in 11 days vs. 10 days. IL-1 and TNF-α release by cultured islets was markedly decreased for op/op islets compared with control islets (IL-1: 0 vs. 4.2 pg/ml, p = 0.07; TNF-α: 67 vs. 311 pg/ml, p = 0.002). In contrast, IL-6 release by op/op islets was significantly increased (11.1 vs. 4.3 ng/ml, p = 0.006). CSF-1-dependent tissue macrophages may not be critical in the inflammatory insult to islet transplants. Alternate patterns of intraislet release of deleterious proinflammatory cytokines may exist.

Original languageEnglish (US)
Pages (from-to)633-637
Number of pages5
JournalCell Transplantation
Volume10
Issue number7
StatePublished - Dec 1 2001

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Keywords

  • Islet primary nonfunction
  • Islet transplantation
  • Macrophages
  • Osteopetrotic mice

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Berney, T., Molano, R. D., Pileggi, A., Cattan, P., Vizzardelli, C., Ricordi, C., & Inverardi, L. (2001). Absence of CSF-1-dependent macrophages does not improve function of transplanted islets of Langerhans. Cell Transplantation, 10(7), 633-637.