Abnormalities expressed in long-term cultures of dorsal root ganglia from the dystrophic mouse

Eiko Okada, Richard P. Bunge, Mary B Bunge

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have compared the development in long-term tissue culture of dorsal root ganglia taken from normal and dystrophic mice. Cultures were prepared from late fetal (15-20 days) or neonatal mice of either the C57BL/6 dy2j/dy2j dystrophic(dy) or C57BL/6J +/+ (control) strain and maintained until fully myelinated (5 weeks or more). Analysis by light and electron microscopy indicated tha the substantial ensheathment failure present in certain dy nerve roots in vivo is not expressed in cultures; myelination and Schwann cell numbers are comparable to control cultures. On the other hand, many of the subtle abnormalities more recently described in distal parts of peripheral nerves of dystrophic mice are expressed in the dy cultures. These include: (a) discontinuity in the basal lamina surrounding both myelin-forming and non-myelinating Schwann cells; (b) elongated nodes of Ranvier occurring along otherwise well myelinated nerve fibres; (c) relatively short myelin internodes that are increased in thickness as well as irregularities of internodes length along a nerve fiber; (d) Schwann cell nuclei substantially displaced from the central point of myelin internodes; and (e) occasional regions of incomplete ensheathment of unmyelinated nerve fibers. In discussing these observations, we present arguments that the dy nerve lesion may be explained by the presence of an abnormality in the extracellular matrix of the peripheral nerve tissues of the dy mouse.

Original languageEnglish
Pages (from-to)455-470
Number of pages16
JournalBrain Research
Volume194
Issue number2
DOIs
StatePublished - Aug 4 1980
Externally publishedYes

Fingerprint

Schwann Cells
Spinal Ganglia
Myelin Sheath
Peripheral Nerves
Ranvier's Nodes
Myelinated Nerve Fibers
Nerve Tissue
Unmyelinated Nerve Fibers
Cell Nucleus
Inbred C57BL Mouse
Nerve Fibers
Basement Membrane
Extracellular Matrix
Electron Microscopy
Cell Count
Light

Keywords

  • basal
  • extracellular matrix
  • lamina
  • muscular dystrophy
  • Schwann cells

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Abnormalities expressed in long-term cultures of dorsal root ganglia from the dystrophic mouse. / Okada, Eiko; Bunge, Richard P.; Bunge, Mary B.

In: Brain Research, Vol. 194, No. 2, 04.08.1980, p. 455-470.

Research output: Contribution to journalArticle

Okada, Eiko ; Bunge, Richard P. ; Bunge, Mary B. / Abnormalities expressed in long-term cultures of dorsal root ganglia from the dystrophic mouse. In: Brain Research. 1980 ; Vol. 194, No. 2. pp. 455-470.
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