Abnormal binding pattern and composition of the NF-κB complex components are involved in increased TNF-α production by tumor bearer B cells

Vijaya I. Charyulu, Diana M Lopez

Research output: Contribution to journalArticle

Abstract

B lymphocytes from mammary tumor bearers are cytotoxic against tumor targets and produce TNF-α. A greater stability of RNA and a decreased rate of RNA degradation was observed in B cells of tumor bearers compared to those of normal mice. The TNF-α promoter contains regions that bind NF-κB, which regulate the rate of transcription. Supershift assays for the NF-κB region showed that there are p50-p65 heterodimers and p50 homodimers in the nuclear extracts of the two types of B cells, while those from tumor bearers lack the c-Rel component that is present in normal B cells. These results indicate that abnormalities in binding and composition of, the NF-κB complexes may be involved in the increased TNF-α production by B cells of tumor bearers.

Original languageEnglish
Pages (from-to)411-416
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume3
Issue number4
StatePublished - Dec 1 1999

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B-Lymphocytes
RNA Stability
Neoplasms
Genetic Promoter Regions
Breast Neoplasms

Keywords

  • B cells
  • C-Rel role
  • Cytokine regulation
  • Mammary tumor bearers
  • TNF-α

ASJC Scopus subject areas

  • Genetics

Cite this

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AB - B lymphocytes from mammary tumor bearers are cytotoxic against tumor targets and produce TNF-α. A greater stability of RNA and a decreased rate of RNA degradation was observed in B cells of tumor bearers compared to those of normal mice. The TNF-α promoter contains regions that bind NF-κB, which regulate the rate of transcription. Supershift assays for the NF-κB region showed that there are p50-p65 heterodimers and p50 homodimers in the nuclear extracts of the two types of B cells, while those from tumor bearers lack the c-Rel component that is present in normal B cells. These results indicate that abnormalities in binding and composition of, the NF-κB complexes may be involved in the increased TNF-α production by B cells of tumor bearers.

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