Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration

Siddhesh Aras, Hassan Arrabi, Neeraja Purandare, Maik Hüttemann, John Kamholz, Stephan L Zuchner, Lawrence I. Grossman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We previously showed that MNRR1 (Mitochondrial Nuclear Retrograde Regulator 1, also CHCHD2) functions in two subcellular compartments, displaying a different function in each. In the mitochondria it is a stress regulator of respiration that binds to cytochrome c oxidase (COX) whereas in the nucleus it is a transactivator of COX4I2 and other hypoxia-stimulated genes. We now show that binding of MNRR1 to COX is promoted by phosphorylation at tyrosine-99 and that this interaction stimulates respiration. We show that phosphorylation of MNRR1 takes place in mitochondria and is mediated by Abl2 kinase (ARG). A family with Charcot-Marie-Tooth disease type 1A with an exaggerated phenotype harbors a Q112H mutation in MNRR1, located in a domain that is necessary for transcriptional activation by MNRR1. Furthermore, the mutation causes the protein to function suboptimally in the mitochondria in response to cellular stress. The Q112H mutation hinders the ability of the protein to interact with Abl kinase, leading to defective tyrosine phosphorylation and a resultant defect in respiration.

Original languageEnglish (US)
Pages (from-to)440-448
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1864
Issue number2
DOIs
StatePublished - Feb 1 2017

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Keywords

  • ABL tyrosine kinase
  • Abl2
  • Mitochondria
  • Nucleus
  • Phosphotyrosine
  • Transcription promoter

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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