Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus

Amitinder Kaur, Hannah B. Sanford, Deirdre Garry, Sabine Lang, Sherry A. Klumpp, Daisuke Watanabe, Roderick T. Bronson, Jeffrey D. Lifson, Margherita Rosati, George N. Pavlakis, Barbara K. Felber, David M. Knipe, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value < 0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value < 0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS.

Original languageEnglish (US)
Pages (from-to)199-214
Number of pages16
JournalVirology
Volume357
Issue number2
DOIs
StatePublished - Jan 20 2007
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
Macaca
Simplexvirus
DNA
Enzyme-Linked Immunospot Assay
vif Gene Products
nef Gene Products
Defective Viruses
AIDS Vaccines
Recombinant DNA
Herpesviridae
Viremia
Neutralizing Antibodies
Macaca mulatta
Vaccines
RNA

Keywords

  • AIDS vaccine
  • DNA prime
  • Recombinant HSV vectors
  • Rhesus macaque
  • SIV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus. / Kaur, Amitinder; Sanford, Hannah B.; Garry, Deirdre; Lang, Sabine; Klumpp, Sherry A.; Watanabe, Daisuke; Bronson, Roderick T.; Lifson, Jeffrey D.; Rosati, Margherita; Pavlakis, George N.; Felber, Barbara K.; Knipe, David M.; Desrosiers, Ronald Charles.

In: Virology, Vol. 357, No. 2, 20.01.2007, p. 199-214.

Research output: Contribution to journalArticle

Kaur, A, Sanford, HB, Garry, D, Lang, S, Klumpp, SA, Watanabe, D, Bronson, RT, Lifson, JD, Rosati, M, Pavlakis, GN, Felber, BK, Knipe, DM & Desrosiers, RC 2007, 'Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus', Virology, vol. 357, no. 2, pp. 199-214. https://doi.org/10.1016/j.virol.2006.08.007
Kaur, Amitinder ; Sanford, Hannah B. ; Garry, Deirdre ; Lang, Sabine ; Klumpp, Sherry A. ; Watanabe, Daisuke ; Bronson, Roderick T. ; Lifson, Jeffrey D. ; Rosati, Margherita ; Pavlakis, George N. ; Felber, Barbara K. ; Knipe, David M. ; Desrosiers, Ronald Charles. / Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus. In: Virology. 2007 ; Vol. 357, No. 2. pp. 199-214.
@article{dc4b89ebdb5f448da6072bcc8acf889b,
title = "Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus",
abstract = "The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value < 0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value < 0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS.",
keywords = "AIDS vaccine, DNA prime, Recombinant HSV vectors, Rhesus macaque, SIV",
author = "Amitinder Kaur and Sanford, {Hannah B.} and Deirdre Garry and Sabine Lang and Klumpp, {Sherry A.} and Daisuke Watanabe and Bronson, {Roderick T.} and Lifson, {Jeffrey D.} and Margherita Rosati and Pavlakis, {George N.} and Felber, {Barbara K.} and Knipe, {David M.} and Desrosiers, {Ronald Charles}",
year = "2007",
month = "1",
day = "20",
doi = "10.1016/j.virol.2006.08.007",
language = "English (US)",
volume = "357",
pages = "199--214",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus

AU - Kaur, Amitinder

AU - Sanford, Hannah B.

AU - Garry, Deirdre

AU - Lang, Sabine

AU - Klumpp, Sherry A.

AU - Watanabe, Daisuke

AU - Bronson, Roderick T.

AU - Lifson, Jeffrey D.

AU - Rosati, Margherita

AU - Pavlakis, George N.

AU - Felber, Barbara K.

AU - Knipe, David M.

AU - Desrosiers, Ronald Charles

PY - 2007/1/20

Y1 - 2007/1/20

N2 - The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value < 0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value < 0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS.

AB - The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value < 0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value < 0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS.

KW - AIDS vaccine

KW - DNA prime

KW - Recombinant HSV vectors

KW - Rhesus macaque

KW - SIV

UR - http://www.scopus.com/inward/record.url?scp=33751399952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751399952&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2006.08.007

DO - 10.1016/j.virol.2006.08.007

M3 - Article

C2 - 16962628

AN - SCOPUS:33751399952

VL - 357

SP - 199

EP - 214

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -