Abstract
Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.
| Original language | English |
|---|---|
| Pages (from-to) | 126-133 |
| Number of pages | 8 |
| Journal | Neurobiology of Disease |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2005 |
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Keywords
- Amyotrophic lateral sclerosis
- Apoptosis
- Cytochrome c
- Immunohistochemistry
- Neurological disorders
- Neuronal death
- SOD1 mice
ASJC Scopus subject areas
- Neurology
Cite this
Aberrant δPKC activation in the spinal cord of Wobbler mouse : A model of motor neuron disease. / Dave, Kunjan R; Raval, Ami; Purroy, Jesús; Kirkinezos, Ilias G.; Moraes, Carlos T; Bradley, Walter G; Perez-Pinzon, Miguel.
In: Neurobiology of Disease, Vol. 18, No. 1, 01.02.2005, p. 126-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aberrant δPKC activation in the spinal cord of Wobbler mouse
T2 - A model of motor neuron disease
AU - Dave, Kunjan R
AU - Raval, Ami
AU - Purroy, Jesús
AU - Kirkinezos, Ilias G.
AU - Moraes, Carlos T
AU - Bradley, Walter G
AU - Perez-Pinzon, Miguel
PY - 2005/2/1
Y1 - 2005/2/1
N2 - Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.
AB - Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.
KW - Amyotrophic lateral sclerosis
KW - Apoptosis
KW - Cytochrome c
KW - Immunohistochemistry
KW - Neurological disorders
KW - Neuronal death
KW - SOD1 mice
UR - http://www.scopus.com/inward/record.url?scp=11844306671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11844306671&partnerID=8YFLogxK
U2 - 10.1016/j.nbd.2004.08.017
DO - 10.1016/j.nbd.2004.08.017
M3 - Article
C2 - 15649703
AN - SCOPUS:11844306671
VL - 18
SP - 126
EP - 133
JO - Neurobiology of Disease
JF - Neurobiology of Disease
SN - 0969-9961
IS - 1
ER -