A waterborne chemical cue from Gulf toadfish, Opsanus beta, prompts pulsatile urea excretion in conspecifics

Jeremy Fulton, Christophe M.R. LeMoine, Carol Bucking, Kevin V. Brix, Patrick J. Walsh, M. Danielle McDonald

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The Gulf toadfish (Opsanus beta) has a fully functional ornithine urea cycle (O-UC) that allows it to excrete nitrogenous waste in the form of urea. Interestingly, urea is excreted in a pulse across the gill that lasts 1–3 h and occurs once or twice a day. Both the stress hormone, cortisol, and the neurotransmitter, serotonin (5-HT) are involved in the control of pulsatile urea excretion. This and other evidence suggests that urea pulsing may be linked to toadfish social behavior. The hypothesis of the present study was that toadfish urea pulses can be triggered by waterborne chemical cues from conspecifics. Our findings indicate that exposure to seawater that held a donor conspecific for up to 48 h (pre-conditioned seawater; PC-SW) induced a urea pulse within 7 h in naïve conspecifics compared to a pulse latency of 20 h when exposed to seawater alone. Factors such as PC-SW intensity and donor body mass influenced the pulse latency response of naïve conspecifics. Fractionation and heat treatment of PC-SW to narrow possible signal candidates revealed that the active chemical was both water-soluble and heat-stable. Fish exposed to urea, cortisol or 5-HT in seawater did not have a pulse latency that was significantly different than seawater alone; however, ammonia, perhaps in the form of NH4Cl, was found to be a factor in the pulse latency response of toadfish to PC-SW and could be one component of a multi-component cue used for chemical communication in toadfish. Further studies are needed to fully identify the chemical cue as well as determine its adaptive significance in this marine teleost fish.

Original languageEnglish (US)
Pages (from-to)92-99
Number of pages8
JournalPhysiology AND Behavior
Volume171
DOIs
StatePublished - Mar 15 2017

Keywords

  • Ammonia
  • Chemical communication
  • Cortisol
  • Nitrogen excretion
  • Serotonin
  • Social/territorial signaling
  • Urea
  • Ureotely

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Fingerprint

Dive into the research topics of 'A waterborne chemical cue from Gulf toadfish, Opsanus beta, prompts pulsatile urea excretion in conspecifics'. Together they form a unique fingerprint.

Cite this