A virus-like agent associated with neurofibromatosis in damselfish

Michael C Schmale, Patrick Gibbs, C. E. Campbell

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Damselfish neurofibromatosis (DNF) is a transmissible disease involving neurofibromas and chromatophoromas affecting bicolor damselfish Stegastes partitus on Florida reefs. Analysis of genomic DNA by Southern blotting techniques demonstrated the presence of a group of extrachromosomal DNAs in tumors from fish affected with DNF but not in healthy individuals. Cell lines obtained from tumors contained identical DNAs and were shown to be tumorigenic in vivo, while lines established from healthy fish did not contain such DNA and were not tumorigenic. These DNA patterns were also observed in experimentally induced tumors. A DNase resistant component of this DNA was isolated from both tumor cells and conditioned media of tumor cell lines suggesting that these sequences were encapsulated in viral particles. These data support the hypothesis that one or more of these extrachromosomal DNA forms is the genome of an unusual virus and that this virus is the etiologic agent of DNF. We have tentatively termed this agent the damselfish virus-like agent (DVLA).

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalDiseases of Aquatic Organisms
Volume49
Issue number2
StatePublished - May 10 2002

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Pomacentridae
virus
tumor
DNA
viruses
neoplasms
cell lines
deoxyribonucleases
fish
virion
Southern blotting
reefs
genomics
reef
genome

Keywords

  • Chromatophore
  • Damselfish
  • Neurofibroma
  • Schwann Cell
  • Tumor
  • Virus

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

A virus-like agent associated with neurofibromatosis in damselfish. / Schmale, Michael C; Gibbs, Patrick; Campbell, C. E.

In: Diseases of Aquatic Organisms, Vol. 49, No. 2, 10.05.2002, p. 107-115.

Research output: Contribution to journalArticle

Schmale, Michael C ; Gibbs, Patrick ; Campbell, C. E. / A virus-like agent associated with neurofibromatosis in damselfish. In: Diseases of Aquatic Organisms. 2002 ; Vol. 49, No. 2. pp. 107-115.
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