A universal plasmid library encoding all permutations of small interfering RNA

Meihong Chen, Lishu Zhang, Hong Yan Zhang, Xiahui Xiong, Bo Wang, Quan Du, Bing Lu, Claes R Wahlestedt, Zicai Liang

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Small interfering RNA (siRNA) is normally designed to silence preselected known genes. Such selections are inevitably prone to bias as a result of limited knowledge about the biological process, transcript identity, and functions. A library that contains all permutations of siRNA could avoid such problems. In this paper, it is shown that 5 × 107 siRNA-encoding plasmids can be constructed in a single tube by using vectors with two mutated RNA polymerase III promoters arranged in a convergent manner. Such a library was used to carry out genomewide screening of functional genes in a phenotype-driven manner. Multiple siRNAs that induce a significant increase of cell proliferation speed were identified.

Original languageEnglish
Pages (from-to)2356-2361
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number7
DOIs
StatePublished - Feb 15 2005
Externally publishedYes

Fingerprint

Small Interfering RNA
Plasmids
Libraries
RNA Polymerase III
Biological Phenomena
Genes
Cell Proliferation
Phenotype

Keywords

  • Dual promoters
  • Random targeting
  • Screening
  • Vector

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A universal plasmid library encoding all permutations of small interfering RNA. / Chen, Meihong; Zhang, Lishu; Zhang, Hong Yan; Xiong, Xiahui; Wang, Bo; Du, Quan; Lu, Bing; Wahlestedt, Claes R; Liang, Zicai.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 7, 15.02.2005, p. 2356-2361.

Research output: Contribution to journalArticle

Chen, Meihong ; Zhang, Lishu ; Zhang, Hong Yan ; Xiong, Xiahui ; Wang, Bo ; Du, Quan ; Lu, Bing ; Wahlestedt, Claes R ; Liang, Zicai. / A universal plasmid library encoding all permutations of small interfering RNA. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 7. pp. 2356-2361.
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