A unique deletion in pBR322 DNA caused by insertion of poly(dA) · poly(dT)

Emeka V. Ikpeazu, Manuel S. Valenzuela

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A unique deletion covering around 43% of the pBR322 genome was found after attempting to insert 100 or 200 bp poly(dA) · poly(dT) into the EcoRV site of pBR322 DNA. This result was not observed if an equivalent size heterologous DNA or a larger poly(dA) · poly(dT) fragment of 10-20,000 bp was introduced at the same site. DNA sequencing analysis at the junctions suggests that a specific intramolecular pairing may be involved in the formation of this deletion mutant.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalPlasmid
Volume21
Issue number3
DOIs
StatePublished - May 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology

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