A unique deletion in pBR322 DNA caused by insertion of poly(dA) · poly(dT)

Chukwuemeka Ikpeazu, Manuel S. Valenzuela

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A unique deletion covering around 43% of the pBR322 genome was found after attempting to insert 100 or 200 bp poly(dA) · poly(dT) into the EcoRV site of pBR322 DNA. This result was not observed if an equivalent size heterologous DNA or a larger poly(dA) · poly(dT) fragment of 10-20,000 bp was introduced at the same site. DNA sequencing analysis at the junctions suggests that a specific intramolecular pairing may be involved in the formation of this deletion mutant.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalPlasmid
Volume21
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

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DNA
DNA Sequence Analysis
Genome
polydeoxyadenylic acid-polythymidylic acid

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A unique deletion in pBR322 DNA caused by insertion of poly(dA) · poly(dT). / Ikpeazu, Chukwuemeka; Valenzuela, Manuel S.

In: Plasmid, Vol. 21, No. 3, 1989, p. 247-250.

Research output: Contribution to journalArticle

Ikpeazu, Chukwuemeka ; Valenzuela, Manuel S. / A unique deletion in pBR322 DNA caused by insertion of poly(dA) · poly(dT). In: Plasmid. 1989 ; Vol. 21, No. 3. pp. 247-250.
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