A two-dimensional protein map of Caenorhabditis elegans

Sabine P. Schrimpf, Hanno Langen, Ana Vaz Gomes, Claes R Wahlestedt

Research output: Contribution to journalArticle

Abstract

A protein map of Caenorhabditis elegans was constructed by using two-dimensional gel electrophoresis followed by peptide mass fingerprinting. A whole worm extract of a mixed population was separated on immobilized pH gradient strips 4-7 L, 3-10 NL, 6-11 L and 12% sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) gels. Gels were stained with colloidal Coomassie blue and 286 spots representing 152 proteins were subsequently identified by matrix-assisted laser desorption/ionization-mass spectrometry after in-gel digestion with trypsin. Most of the identified proteins with known cellular function were enzymes related to carbohydrate and lipid metabolism, or structural proteins with subcellular locations in the cytoplasm, mitochondria or cytoskeleton.

Original languageEnglish
Pages (from-to)1224-1232
Number of pages9
JournalElectrophoresis
Volume22
Issue number6
DOIs
StatePublished - May 17 2001
Externally publishedYes

Fingerprint

Caenorhabditis elegans Proteins
Gels
Proteins
Proton-Motive Force
Peptide Mapping
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Carbohydrate Metabolism
Cytoskeleton
Lipid Metabolism
Sodium Dodecyl Sulfate
Trypsin
Mitochondria
Digestion
Cytoplasm
Electrophoresis
Ionization
Mass spectrometry
Desorption
Enzymes

Keywords

  • Caenorhabditis elegans
  • Peptide mass fingerprinting
  • Protein map
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

A two-dimensional protein map of Caenorhabditis elegans. / Schrimpf, Sabine P.; Langen, Hanno; Gomes, Ana Vaz; Wahlestedt, Claes R.

In: Electrophoresis, Vol. 22, No. 6, 17.05.2001, p. 1224-1232.

Research output: Contribution to journalArticle

Schrimpf, Sabine P. ; Langen, Hanno ; Gomes, Ana Vaz ; Wahlestedt, Claes R. / A two-dimensional protein map of Caenorhabditis elegans. In: Electrophoresis. 2001 ; Vol. 22, No. 6. pp. 1224-1232.
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