Abstract
The neuritic plaques formed by amyloid β-peptide (Aβ) play a seminal role in the pathogenesis of Alzheimer's disease (AD). Aβ sequence 25-35 (GSNKGAIIGLM) is among the most frequently studied Aβ derivatives for the reason that it possesses the structural characteristic of Aβ and remains neurotoxic. Aβ(25-35) was modified with an aliphatic chain (C 18) at the N-terminal of the peptide for the study of the Langmuir monolayer at the air-water interface. The main advantage of the 2D approach is the self-assembly of the peptide moiety in the subphase, and therefore the aggregation process of the peptidolipid Aβ(25-35) was monitored by surface pressure and surface potential-area isotherms. The real-time epifluorescence microscopy was utilized to observe the topography of the domains formed, whereas polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) provided the information on the structural features of the domains at the air-water interface. Langmuir-Blodgett films were prepared to examine by circular dichroism (CD) the conformation of the peptidolipid film in the domains.
Original language | English (US) |
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Pages (from-to) | 4661-4666 |
Number of pages | 6 |
Journal | Journal of Physical Chemistry C |
Volume | 111 |
Issue number | 12 |
DOIs | |
State | Published - Mar 29 2007 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Energy(all)
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films