A two-dimensional approach to study amyloid β-peptide fragment (25-35)

Robert C. Triulzi, Changqing Li, David Naistat, Jhony Orbulescu, Roger Leblanc

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The neuritic plaques formed by amyloid β-peptide (Aβ) play a seminal role in the pathogenesis of Alzheimer's disease (AD). Aβ sequence 25-35 (GSNKGAIIGLM) is among the most frequently studied Aβ derivatives for the reason that it possesses the structural characteristic of Aβ and remains neurotoxic. Aβ(25-35) was modified with an aliphatic chain (C 18) at the N-terminal of the peptide for the study of the Langmuir monolayer at the air-water interface. The main advantage of the 2D approach is the self-assembly of the peptide moiety in the subphase, and therefore the aggregation process of the peptidolipid Aβ(25-35) was monitored by surface pressure and surface potential-area isotherms. The real-time epifluorescence microscopy was utilized to observe the topography of the domains formed, whereas polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) provided the information on the structural features of the domains at the air-water interface. Langmuir-Blodgett films were prepared to examine by circular dichroism (CD) the conformation of the peptidolipid film in the domains.

Original languageEnglish
Pages (from-to)4661-4666
Number of pages6
JournalJournal of Physical Chemistry C
Volume111
Issue number12
DOIs
StatePublished - Mar 29 2007

Fingerprint

Peptide Fragments
Amyloid
Peptides
peptides
fragments
Water
Langmuir Blodgett films
Air
Absorption spectroscopy
Self assembly
Topography
Isotherms
Conformations
Monolayers
pathogenesis
polarization modulation
Microscopic examination
Agglomeration
monomolecular films
infrared reflection

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

A two-dimensional approach to study amyloid β-peptide fragment (25-35). / Triulzi, Robert C.; Li, Changqing; Naistat, David; Orbulescu, Jhony; Leblanc, Roger.

In: Journal of Physical Chemistry C, Vol. 111, No. 12, 29.03.2007, p. 4661-4666.

Research output: Contribution to journalArticle

Triulzi, Robert C. ; Li, Changqing ; Naistat, David ; Orbulescu, Jhony ; Leblanc, Roger. / A two-dimensional approach to study amyloid β-peptide fragment (25-35). In: Journal of Physical Chemistry C. 2007 ; Vol. 111, No. 12. pp. 4661-4666.
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