A tractable, simplified ex vivo human skin model of wound infection

Daniel J. Yoon, Daniel R. Fregoso, Duc Nguyen, Vivien Chen, Natasa Strbo, Jaime J. Fuentes, Marjana Tomic-Canic, Robert Crawford, Irena Pastar, R. Rivkah Isseroff

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The prevalence of infection in chronic wounds is well documented in the literature but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired reepithelialization compared to the control. Additionally, promigratory and proreparative genes were significantly downregulated, while proinflammatory genes were significantly upregulated, demonstrating molecular characterizations of impaired healing as in chronic wounds. This model allows for a simplified and versatile tool for the study of wound infection and subsequent development of novel therapies.

Original languageEnglish (US)
JournalWound Repair and Regeneration
DOIs
StatePublished - Jan 1 2019

Fingerprint

Wound Infection
Skin
Wounds and Injuries
Biofilms
Infection
Cosmetics
Genes
Staphylococcus aureus
Down-Regulation
Costs and Cost Analysis
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Yoon, D. J., Fregoso, D. R., Nguyen, D., Chen, V., Strbo, N., Fuentes, J. J., ... Isseroff, R. R. (2019). A tractable, simplified ex vivo human skin model of wound infection. Wound Repair and Regeneration. https://doi.org/10.1111/wrr.12712

A tractable, simplified ex vivo human skin model of wound infection. / Yoon, Daniel J.; Fregoso, Daniel R.; Nguyen, Duc; Chen, Vivien; Strbo, Natasa; Fuentes, Jaime J.; Tomic-Canic, Marjana; Crawford, Robert; Pastar, Irena; Isseroff, R. Rivkah.

In: Wound Repair and Regeneration, 01.01.2019.

Research output: Contribution to journalArticle

Yoon, Daniel J. ; Fregoso, Daniel R. ; Nguyen, Duc ; Chen, Vivien ; Strbo, Natasa ; Fuentes, Jaime J. ; Tomic-Canic, Marjana ; Crawford, Robert ; Pastar, Irena ; Isseroff, R. Rivkah. / A tractable, simplified ex vivo human skin model of wound infection. In: Wound Repair and Regeneration. 2019.
@article{e617c1c08b4443c08b7b1a4a84dfa6ee,
title = "A tractable, simplified ex vivo human skin model of wound infection",
abstract = "The prevalence of infection in chronic wounds is well documented in the literature but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired reepithelialization compared to the control. Additionally, promigratory and proreparative genes were significantly downregulated, while proinflammatory genes were significantly upregulated, demonstrating molecular characterizations of impaired healing as in chronic wounds. This model allows for a simplified and versatile tool for the study of wound infection and subsequent development of novel therapies.",
author = "Yoon, {Daniel J.} and Fregoso, {Daniel R.} and Duc Nguyen and Vivien Chen and Natasa Strbo and Fuentes, {Jaime J.} and Marjana Tomic-Canic and Robert Crawford and Irena Pastar and Isseroff, {R. Rivkah}",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/wrr.12712",
language = "English (US)",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - A tractable, simplified ex vivo human skin model of wound infection

AU - Yoon, Daniel J.

AU - Fregoso, Daniel R.

AU - Nguyen, Duc

AU - Chen, Vivien

AU - Strbo, Natasa

AU - Fuentes, Jaime J.

AU - Tomic-Canic, Marjana

AU - Crawford, Robert

AU - Pastar, Irena

AU - Isseroff, R. Rivkah

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The prevalence of infection in chronic wounds is well documented in the literature but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired reepithelialization compared to the control. Additionally, promigratory and proreparative genes were significantly downregulated, while proinflammatory genes were significantly upregulated, demonstrating molecular characterizations of impaired healing as in chronic wounds. This model allows for a simplified and versatile tool for the study of wound infection and subsequent development of novel therapies.

AB - The prevalence of infection in chronic wounds is well documented in the literature but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired reepithelialization compared to the control. Additionally, promigratory and proreparative genes were significantly downregulated, while proinflammatory genes were significantly upregulated, demonstrating molecular characterizations of impaired healing as in chronic wounds. This model allows for a simplified and versatile tool for the study of wound infection and subsequent development of novel therapies.

UR - http://www.scopus.com/inward/record.url?scp=85063578595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063578595&partnerID=8YFLogxK

U2 - 10.1111/wrr.12712

DO - 10.1111/wrr.12712

M3 - Article

C2 - 30825247

AN - SCOPUS:85063578595

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

ER -