A tiling resolution DNA microarray with complete coverage of the human genome

Adrian Ishkanian, Chad A. Malloff, Spencer K. Watson, Ronald J. DeLeeuw, Bryan Chi, Bradley P. Coe, Antoine Snijders, Donna G. Albertson, Daniel Pinkel, Marco A. Marra, Victor Ling, Calum MacAulay, Wan L. Lam

Research output: Contribution to journalArticle

520 Citations (Scopus)

Abstract

We constructed a tiling resolution array consisting of 32,433 overlapping BAC clones covering the entire human genome. This increases our ability to identify genetic alterations and their boundaries throughout the genome in a single comparative genomic hybridization (CGH) experiment. At this tiling resolution, we identified minute DNA alterations not previously reported. These alterations include microamplifications and deletions containing oncogenes, tumor-suppressor genes and new genes that may be associated with multiple tumor types. Our findings show the need to move beyond conventional marker-based genome comparison approaches, that rely on inference of continuity between interval markers. Our submegabase resolution tiling set for array CGH (SMRT array) allows comprehensive assessment of genomic integrity and thereby the identification of new genes associated with disease.

Original languageEnglish (US)
Pages (from-to)299-303
Number of pages5
JournalNature Genetics
Volume36
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

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Comparative Genomic Hybridization
Human Genome
Oligonucleotide Array Sequence Analysis
Genome
Tumor Suppressor Genes
Oncogenes
Genes
Clone Cells
DNA
Neoplasms

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Ishkanian, A., Malloff, C. A., Watson, S. K., DeLeeuw, R. J., Chi, B., Coe, B. P., ... Lam, W. L. (2004). A tiling resolution DNA microarray with complete coverage of the human genome. Nature Genetics, 36(3), 299-303. https://doi.org/10.1038/ng1307

A tiling resolution DNA microarray with complete coverage of the human genome. / Ishkanian, Adrian; Malloff, Chad A.; Watson, Spencer K.; DeLeeuw, Ronald J.; Chi, Bryan; Coe, Bradley P.; Snijders, Antoine; Albertson, Donna G.; Pinkel, Daniel; Marra, Marco A.; Ling, Victor; MacAulay, Calum; Lam, Wan L.

In: Nature Genetics, Vol. 36, No. 3, 03.2004, p. 299-303.

Research output: Contribution to journalArticle

Ishkanian, A, Malloff, CA, Watson, SK, DeLeeuw, RJ, Chi, B, Coe, BP, Snijders, A, Albertson, DG, Pinkel, D, Marra, MA, Ling, V, MacAulay, C & Lam, WL 2004, 'A tiling resolution DNA microarray with complete coverage of the human genome', Nature Genetics, vol. 36, no. 3, pp. 299-303. https://doi.org/10.1038/ng1307
Ishkanian A, Malloff CA, Watson SK, DeLeeuw RJ, Chi B, Coe BP et al. A tiling resolution DNA microarray with complete coverage of the human genome. Nature Genetics. 2004 Mar;36(3):299-303. https://doi.org/10.1038/ng1307
Ishkanian, Adrian ; Malloff, Chad A. ; Watson, Spencer K. ; DeLeeuw, Ronald J. ; Chi, Bryan ; Coe, Bradley P. ; Snijders, Antoine ; Albertson, Donna G. ; Pinkel, Daniel ; Marra, Marco A. ; Ling, Victor ; MacAulay, Calum ; Lam, Wan L. / A tiling resolution DNA microarray with complete coverage of the human genome. In: Nature Genetics. 2004 ; Vol. 36, No. 3. pp. 299-303.
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