A third human retinoic acid receptor, hRAR-γ

A. Krust, Ph Kastner, M. Petkovich, A. Zelent, P. Chambon

Research output: Contribution to journalArticlepeer-review

598 Scopus citations

Abstract

Retinoic acid receptors (RARs) are retinoic acid (RA)-inducible enhancer factors belonging to the superfamily of steroid/thyroid nuclear receptors. We have previously characterized two human RAR (hRAR-α and hRAR-β) cDNAs and have recently cloned their murine cognates (mRAR-α and mRAR-β) together with a third RAR (mRAR-γ) whose RNA was detected predominantly in skin, a well-known target for RA. mRAR-γ cDNA was used here to clone its human counterpart (hRAR-γ) from a T47D breast cancer cell cDNA library. Using a transient transfection assay in HeLa cells and a receptor gene harboring a synthetic RA responsive element, we demonstrate that hRAR-γ cDNA indeed encodes a RA-inducible transcriptional trans-activator. Interestingly, comparisons of the amino acid sequences of all six human and mouse RARs indicate that the interspecies conservation of a given number of the RAR subfamily (either α, β, or γ) is much higher than the conservation of all three receptors within a given species. These observations indicate that RAR-α, -β, and -γ may perform specific functions. We show also that hRAR-γ RNA is the predominant RAR RNA species in human skin, which suggests that hRAR-γ mediates some of the retinoid effects in this tissue.

Original languageEnglish (US)
Pages (from-to)5310-5314
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number14
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • General

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