A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by InVivo Imaging in the Mouse Spinal Cord

Ariana O. Lorenzana, Jae Lee, Matthew Mui, Amy Chang, Binhai Zheng

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The complex morphology of axons presents a challenge in understanding axonal responses to injury and disease. By invivo two-photon imaging of spinal dorsal column sensory axons, we systematically examined the effect of injury location relative to the main bifurcation point on axon degeneration and regeneration following highly localized laser injuries. Retrograde but not anterograde degeneration was strongly blocked at the bifurcation point at both the acute and subacute phases. Eliminating either the ascending or descending branch led to a poor regenerative response, while eliminating both led to a strong regenerative response. Thus, a surviving intact branch suppresses both retrograde degeneration and regeneration of the injured branch, thereby preserving the remaining axon architecture. Regenerating axons exhibited a dynamic pattern with alternating phases of regeneration and pruning over a chronic period. Invivo imaging continues to reveal new insights on axonal responses to injury in the mammalian spinal cord.

Original languageEnglish (US)
Pages (from-to)947-954
Number of pages8
JournalNeuron
Volume86
Issue number4
DOIs
StatePublished - May 20 2015

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Axons
Spinal Cord
Wounds and Injuries
Regeneration
Retrograde Degeneration
Photons
Lasers
Spine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by InVivo Imaging in the Mouse Spinal Cord. / Lorenzana, Ariana O.; Lee, Jae; Mui, Matthew; Chang, Amy; Zheng, Binhai.

In: Neuron, Vol. 86, No. 4, 20.05.2015, p. 947-954.

Research output: Contribution to journalArticle

Lorenzana, Ariana O. ; Lee, Jae ; Mui, Matthew ; Chang, Amy ; Zheng, Binhai. / A Surviving Intact Branch Stabilizes Remaining Axon Architecture after Injury as Revealed by InVivo Imaging in the Mouse Spinal Cord. In: Neuron. 2015 ; Vol. 86, No. 4. pp. 947-954.
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