A 31P-magnetic resonance study of antegrade and retrograde cerebral perfusion during aortic arch surgery in pigs

Carlos L. Filgueiras, BeAtrice Winsborrow, Jian Ye, Jack Scott, Alexander Aronov, Piotr Kozlowski, Laleh Shabnavard, Randy Summers, John K. Saunders, Roxanne Deslauriers, Tomas Salerno

Research output: Contribution to journalArticle

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Abstract

To evaluate the effect of hypothermic circulatory arrest on brain metabolism, we used 31P-magnetic resonance spectroscopy to monitor brain metabolites in pigs during 2 hours of ischemia and 1 hour of reperfusion. Twenty-eight pigs were divided into five groups. Anesthesia (n=5) and hypothermic cardiopulmonary bypass groups (n=5) served as controls. In the circulatory arrest (n=6), antegrade perfusion (n=6), and retrograde (n=6) brain perfusion groups, the bypass flow rate was 60 to 100 ml·kg-1·min-1. In the antegrade group, the brain was perfused via the carotid arteries at a blood flow rate of 180 to 200 ml·min-1 during circulatory arrest at 15° C. In the retrograde group, the brain was perfused through the superior vena cava at a flow rate of 300 to 500 ml·min-1 during circulatory arrest at 15° C. The intracellular pH was 7.1±0.1 and 7.3±0.1 in the anesthesia and hypothermic cardiopulmonary bypass groups, respectively. In the circulatory arrest group, the intracellular pH decreased to 6.2±0.1 and did not recover to its initial value (7.0±0.1) during reperfusion (p<0.05 compared with the value obtained from the control groups at the corresponding time). Inorganic phosphate did not return to its initial level during reperfusion. In three animals in this group, levels of high-energy phosphates, adenosine triphosphate and phosphocreatine, recovered partially but did not reach the levels observed before arrest. In the group receiving antegrade perfusion, cerebral metabolites and intracellular pH were unchanged throughout the protocol. During circulatory arrest in the retrograde perfusion group the intracellular pH decreased to 6.4±0.1 and recovered fully during reperfusion (7.1±0.1). High-energy phosphates also returned to their initial levels during reperfusion. These studies show that deep hypothermic circulatory arrest with antegrade brain perfusion provides the best brain protection of the options investigated.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalThe Journal of thoracic and cardiovascular surgery
Volume110
Issue number1
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Thoracic Aorta
Magnetic Resonance Spectroscopy
Swine
Perfusion
Reperfusion
Brain
Phosphates
Cardiopulmonary Bypass
Anesthesia
Deep Hypothermia Induced Circulatory Arrest
Superior Vena Cava
Phosphocreatine
Carotid Arteries
Ischemia
Adenosine Triphosphate
Control Groups

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

A 31P-magnetic resonance study of antegrade and retrograde cerebral perfusion during aortic arch surgery in pigs. / Filgueiras, Carlos L.; Winsborrow, BeAtrice; Ye, Jian; Scott, Jack; Aronov, Alexander; Kozlowski, Piotr; Shabnavard, Laleh; Summers, Randy; Saunders, John K.; Deslauriers, Roxanne; Salerno, Tomas.

In: The Journal of thoracic and cardiovascular surgery, Vol. 110, No. 1, 01.01.1995, p. 55-62.

Research output: Contribution to journalArticle

Filgueiras, CL, Winsborrow, B, Ye, J, Scott, J, Aronov, A, Kozlowski, P, Shabnavard, L, Summers, R, Saunders, JK, Deslauriers, R & Salerno, T 1995, 'A 31P-magnetic resonance study of antegrade and retrograde cerebral perfusion during aortic arch surgery in pigs', The Journal of thoracic and cardiovascular surgery, vol. 110, no. 1, pp. 55-62. https://doi.org/10.1016/S0022-5223(05)80009-1
Filgueiras, Carlos L. ; Winsborrow, BeAtrice ; Ye, Jian ; Scott, Jack ; Aronov, Alexander ; Kozlowski, Piotr ; Shabnavard, Laleh ; Summers, Randy ; Saunders, John K. ; Deslauriers, Roxanne ; Salerno, Tomas. / A 31P-magnetic resonance study of antegrade and retrograde cerebral perfusion during aortic arch surgery in pigs. In: The Journal of thoracic and cardiovascular surgery. 1995 ; Vol. 110, No. 1. pp. 55-62.
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AU - Kozlowski, Piotr

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