A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

R. V. Sairam, G. Franklin, R. Hassel, B. Smith, K. Meeker, Nilesh Kashikar, M. Parani, D. Al Abed, S. Ismail, K. Berry, S. L. Goldman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume75
Issue number1
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Plant Growth Regulators
Organogenesis
Bony Callus
organogenesis
Soybeans
plant growth substances
Regeneration
callus
Genotype
soybeans
sugars
2,4-Dichlorophenoxyacetic Acid
genotype
2,4-D
Carbon
buds
shoots
Maltose
Sorbitol
carbon

Keywords

  • Callus
  • Carbon source
  • Cotyledonary node
  • Organogenesis
  • Soybean

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. / Sairam, R. V.; Franklin, G.; Hassel, R.; Smith, B.; Meeker, K.; Kashikar, Nilesh; Parani, M.; Abed, D. Al; Ismail, S.; Berry, K.; Goldman, S. L.

In: Plant Cell, Tissue and Organ Culture, Vol. 75, No. 1, 01.10.2003, p. 79-85.

Research output: Contribution to journalArticle

Sairam, RV, Franklin, G, Hassel, R, Smith, B, Meeker, K, Kashikar, N, Parani, M, Abed, DA, Ismail, S, Berry, K & Goldman, SL 2003, 'A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus', Plant Cell, Tissue and Organ Culture, vol. 75, no. 1, pp. 79-85. https://doi.org/10.1023/A:1024649122748
Sairam, R. V. ; Franklin, G. ; Hassel, R. ; Smith, B. ; Meeker, K. ; Kashikar, Nilesh ; Parani, M. ; Abed, D. Al ; Ismail, S. ; Berry, K. ; Goldman, S. L. / A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. In: Plant Cell, Tissue and Organ Culture. 2003 ; Vol. 75, No. 1. pp. 79-85.
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