A study of the binding of Graves' immunoglobulins to orbital antigens

K. Banovac, Silvina Levis, S. F. Byrne

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The binding of Graves' immunoglobulins to membranes of human eye muscle (HEM) and guinea pig Harderian gland (HG) were studied. The membrane fraction of 100,000 x g sediment was used for ELISA. Serum samples from 55 patients with Graves' ophthalmopathy were evaluated for binding to the membrane preparation. There was a higher binding to HG with the sera from the patients with Graves' ophthalmopathy than in the control group (p < 0.01), but there was no difference in binding to HEM. Purification of IgG from sera improved binding to HG in both patients' (p < 0.001) and control group (p < 0.005). There was also an increase in percentage of positive responses obtained with IgG 48% vs serum samples 37%. In 23 out of 24 patients we found the thickening of extraocular muscles by A-scan ultrasonography. In these groups of patients and 3 others with malignant ophthalmopathy the binding of IgG preparation to HG was similar to control group. In all assays there was an overlap between patients with Graves' ophthalmopathy and control subjects, and a lack of relationship between the responses in ELISA and clinical or severity of ophthalmopathy.

Original languageEnglish
Pages (from-to)483-488
Number of pages6
JournalJournal of Endocrinological Investigation
Volume11
Issue number7
StatePublished - Jan 1 1988

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Harderian Gland
Immunoglobulins
Graves Ophthalmopathy
Antigens
Immunoglobulin G
Serum
Control Groups
Membranes
Oculomotor Muscles
Enzyme-Linked Immunosorbent Assay
Muscles
Ultrasonography
Guinea Pigs

ASJC Scopus subject areas

  • Endocrinology

Cite this

A study of the binding of Graves' immunoglobulins to orbital antigens. / Banovac, K.; Levis, Silvina; Byrne, S. F.

In: Journal of Endocrinological Investigation, Vol. 11, No. 7, 01.01.1988, p. 483-488.

Research output: Contribution to journalArticle

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