A structural model of transcription elongation

Nataliya Korzheva, Arkady Mustaev, Maxim Kozlov, Arun Malhotra, Vadim Nikiforov, Alex Goldfarb, Seth A. Darst

Research output: Contribution to journalArticle

325 Citations (Scopus)

Abstract

The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the β' subunit and enclosed on top by the β subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the β subunit harboring rifampicin resistance mutations, to the β' subunit 'ruder.' The single-stranded RNA is then extruded through another channel formed by the β-subunit flap domain. The model provides insight into the functional properties of the transcription complex.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalScience
Volume289
Issue number5479
DOIs
StatePublished - Jul 28 2000

Fingerprint

Structural Models
RNA
DNA-Directed RNA Polymerases
DNA
Bacterial RNA
Rifampin
Nucleic Acids
Catalytic Domain
X-Rays
Mutation
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Korzheva, N., Mustaev, A., Kozlov, M., Malhotra, A., Nikiforov, V., Goldfarb, A., & Darst, S. A. (2000). A structural model of transcription elongation. Science, 289(5479), 619-625. https://doi.org/10.1126/science.289.5479.619

A structural model of transcription elongation. / Korzheva, Nataliya; Mustaev, Arkady; Kozlov, Maxim; Malhotra, Arun; Nikiforov, Vadim; Goldfarb, Alex; Darst, Seth A.

In: Science, Vol. 289, No. 5479, 28.07.2000, p. 619-625.

Research output: Contribution to journalArticle

Korzheva, N, Mustaev, A, Kozlov, M, Malhotra, A, Nikiforov, V, Goldfarb, A & Darst, SA 2000, 'A structural model of transcription elongation', Science, vol. 289, no. 5479, pp. 619-625. https://doi.org/10.1126/science.289.5479.619
Korzheva N, Mustaev A, Kozlov M, Malhotra A, Nikiforov V, Goldfarb A et al. A structural model of transcription elongation. Science. 2000 Jul 28;289(5479):619-625. https://doi.org/10.1126/science.289.5479.619
Korzheva, Nataliya ; Mustaev, Arkady ; Kozlov, Maxim ; Malhotra, Arun ; Nikiforov, Vadim ; Goldfarb, Alex ; Darst, Seth A. / A structural model of transcription elongation. In: Science. 2000 ; Vol. 289, No. 5479. pp. 619-625.
@article{589bb67edd3f464dab27e9a04771215d,
title = "A structural model of transcription elongation",
abstract = "The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the β' subunit and enclosed on top by the β subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the β subunit harboring rifampicin resistance mutations, to the β' subunit 'ruder.' The single-stranded RNA is then extruded through another channel formed by the β-subunit flap domain. The model provides insight into the functional properties of the transcription complex.",
author = "Nataliya Korzheva and Arkady Mustaev and Maxim Kozlov and Arun Malhotra and Vadim Nikiforov and Alex Goldfarb and Darst, {Seth A.}",
year = "2000",
month = "7",
day = "28",
doi = "10.1126/science.289.5479.619",
language = "English",
volume = "289",
pages = "619--625",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5479",

}

TY - JOUR

T1 - A structural model of transcription elongation

AU - Korzheva, Nataliya

AU - Mustaev, Arkady

AU - Kozlov, Maxim

AU - Malhotra, Arun

AU - Nikiforov, Vadim

AU - Goldfarb, Alex

AU - Darst, Seth A.

PY - 2000/7/28

Y1 - 2000/7/28

N2 - The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the β' subunit and enclosed on top by the β subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the β subunit harboring rifampicin resistance mutations, to the β' subunit 'ruder.' The single-stranded RNA is then extruded through another channel formed by the β-subunit flap domain. The model provides insight into the functional properties of the transcription complex.

AB - The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the β' subunit and enclosed on top by the β subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the β subunit harboring rifampicin resistance mutations, to the β' subunit 'ruder.' The single-stranded RNA is then extruded through another channel formed by the β-subunit flap domain. The model provides insight into the functional properties of the transcription complex.

UR - http://www.scopus.com/inward/record.url?scp=0034725870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034725870&partnerID=8YFLogxK

U2 - 10.1126/science.289.5479.619

DO - 10.1126/science.289.5479.619

M3 - Article

C2 - 10915625

AN - SCOPUS:0034725870

VL - 289

SP - 619

EP - 625

JO - Science

JF - Science

SN - 0036-8075

IS - 5479

ER -