A structural model for the HIV-1 Rev-RRE complex deduced from altered- specificity Rev variants isolated by a rapid genetic strategy

Chaitanya Jain, Joel G. Belasco

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

A broadly applicable genetic strategy was developed for investigating RNA-protein interactions and applied to the HIV-1 Ray protein. By rapidly screening thousands of Rev-RNA interactions in Escherichia coli, we isolated Rev suppressor mutations that alleviated the deleterious effect of mutations in RRE stem-loop IIB, the high affinity RNA-binding site for Rev. All of these suppressor mutations map to a single arginine-deficient face of a Rev α-helix, and some alter the binding specificity of the protein, providing genetic evidence for direct contacts between specific Rev amino acids and RNA nucleotides in the RNA complex of Rev. The spatial constraints suggested by these data have enabled us to model the structure of this complex.

Original languageEnglish (US)
Pages (from-to)115-125
Number of pages11
JournalCell
Volume87
Issue number1
DOIs
StatePublished - Oct 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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