A stop codon-dependent internal secondary translation initiation region in Escherichia coli rpoS

Pochi Ramalingam Subbarayan, Malancha Sarkar

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Sigma S (σs) encoded by rpoS is a stationary phase-specific σ subunit of the Escherichia coli RNA polymerase holoenzyme. In many E. coli strains, rpoS has an amber stop as codon 33 (rpoSAm), resulting in a 32-amino-acid-long peptide. Nevertheless, suppressor-free rpoSAm strains have functional σS. This led us to hypothesize the presence of an intracistronic secondary translational initiation region (STIR) in the E. coli rpoS gene. Here, we demonstrate that the STIR is functional and is controlled by the upstream amber stop codon 33. Removal of the primary translational initiation region did not abolish translation from STIR, ruling out translational coupling. Importantly, the functional STIR conferred survival advantage. Taken together, our results reveal a hitherto unknown physiologically significant post-transcriptional process in E. coli rpoSAm strains.

Original languageEnglish (US)
Pages (from-to)1359-1365
Number of pages7
JournalRNA
Volume10
Issue number9
DOIs
StatePublished - Sep 2004

Keywords

  • σ
  • GASP
  • Intracistronic translation initiation
  • Ribosome binding site
  • rpoS
  • STIR

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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