A species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish

Tamzin A. Blewett, Tiffany L. Chow, Deborah L. Maclatchy, Chris M. Wood

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Differences exist among fish species in their sensitivity to endocrine disruptors such as 17-α-ethynylestradiol (EE2). We examined whether there were corresponding differences in EE2 uptake rates and short-term internal distribution patterns. Six freshwater species: Japanese ricefish (medaka, Oryzias latipes), goldfish (Carassius auratus), zebrafish (Danio rerio), fathead minnow (Pimephales promelas), Atlantic killifish (Fundulus heteroclitus) and rainbow trout (Oncorhynchus mykiss) were exposed to waterborne radiolabelled EE2 (100 ng/L) for 2-h measurements of uptake, tissue accumulation and oxygen consumption rates (MO2). EE2 uptake rate and MO2 were relatively consistent among species (2.5-3.0 fold variation), with the only significant differences being a lower EE2 uptake rate in medaka, and lower MO2 in medaka, goldfish, and zebrafish relative to the other species. EE2 accumulation, however, exhibited two distinct patterns, suggesting differences in metabolic processing. In killifish and medaka, the highest accumulation (~ 50%) occurred in the liver and gallbladder, whereas in minnow, goldfish, zebrafish and trout, > 50% accumulated in the carcass. No significant sex differences were found in killifish or minnow, apart from lower gill tissue EE2 accumulation in minnow females. This study demonstrated that metabolic processing of EE2 may be species-specific and tissue specific EE2 distribution profiles vary. These could be indicative of differences in overall EE2 sensitivity among species.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume161
Issue number1
DOIs
StatePublished - Apr 1 2014

Fingerprint

Oryzias
Tissue Distribution
Cyprinidae
Goldfish
Fish
Fundulidae
Zebrafish
Fishes
Tissue
Oncorhynchus mykiss
Endocrine Disruptors
Processing
Liver
Trout
Oxygen
Gallbladder
Fresh Water
Oxygen Consumption
Sex Characteristics

Keywords

  • 17α-Ethynylestradiol (EE2)
  • Fathead minnow
  • Goldfish
  • Japanese medaka
  • Killifish
  • Rainbow trout
  • Tissue distribution
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

A species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish. / Blewett, Tamzin A.; Chow, Tiffany L.; Maclatchy, Deborah L.; Wood, Chris M.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 161, No. 1, 01.04.2014, p. 33-40.

Research output: Contribution to journalArticle

Blewett, Tamzin A. ; Chow, Tiffany L. ; Maclatchy, Deborah L. ; Wood, Chris M. / A species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish. In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2014 ; Vol. 161, No. 1. pp. 33-40.
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