Abstract
Differences exist among fish species in their sensitivity to endocrine disruptors such as 17-α-ethynylestradiol (EE2). We examined whether there were corresponding differences in EE2 uptake rates and short-term internal distribution patterns. Six freshwater species: Japanese ricefish (medaka, Oryzias latipes), goldfish (Carassius auratus), zebrafish (Danio rerio), fathead minnow (Pimephales promelas), Atlantic killifish (Fundulus heteroclitus) and rainbow trout (Oncorhynchus mykiss) were exposed to waterborne radiolabelled EE2 (100 ng/L) for 2-h measurements of uptake, tissue accumulation and oxygen consumption rates (MO2). EE2 uptake rate and MO2 were relatively consistent among species (2.5-3.0 fold variation), with the only significant differences being a lower EE2 uptake rate in medaka, and lower MO2 in medaka, goldfish, and zebrafish relative to the other species. EE2 accumulation, however, exhibited two distinct patterns, suggesting differences in metabolic processing. In killifish and medaka, the highest accumulation (~ 50%) occurred in the liver and gallbladder, whereas in minnow, goldfish, zebrafish and trout, > 50% accumulated in the carcass. No significant sex differences were found in killifish or minnow, apart from lower gill tissue EE2 accumulation in minnow females. This study demonstrated that metabolic processing of EE2 may be species-specific and tissue specific EE2 distribution profiles vary. These could be indicative of differences in overall EE2 sensitivity among species.
| Original language | English |
|---|---|
| Pages (from-to) | 33-40 |
| Number of pages | 8 |
| Journal | Comparative Biochemistry and Physiology - C Toxicology and Pharmacology |
| Volume | 161 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 1 2014 |
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Keywords
- 17α-Ethynylestradiol (EE2)
- Fathead minnow
- Goldfish
- Japanese medaka
- Killifish
- Rainbow trout
- Tissue distribution
- Zebrafish
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Physiology
- Health, Toxicology and Mutagenesis
- Toxicology
Cite this
A species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish. / Blewett, Tamzin A.; Chow, Tiffany L.; Maclatchy, Deborah L.; Wood, Chris M.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 161, No. 1, 01.04.2014, p. 33-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish
AU - Blewett, Tamzin A.
AU - Chow, Tiffany L.
AU - Maclatchy, Deborah L.
AU - Wood, Chris M.
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Differences exist among fish species in their sensitivity to endocrine disruptors such as 17-α-ethynylestradiol (EE2). We examined whether there were corresponding differences in EE2 uptake rates and short-term internal distribution patterns. Six freshwater species: Japanese ricefish (medaka, Oryzias latipes), goldfish (Carassius auratus), zebrafish (Danio rerio), fathead minnow (Pimephales promelas), Atlantic killifish (Fundulus heteroclitus) and rainbow trout (Oncorhynchus mykiss) were exposed to waterborne radiolabelled EE2 (100 ng/L) for 2-h measurements of uptake, tissue accumulation and oxygen consumption rates (MO2). EE2 uptake rate and MO2 were relatively consistent among species (2.5-3.0 fold variation), with the only significant differences being a lower EE2 uptake rate in medaka, and lower MO2 in medaka, goldfish, and zebrafish relative to the other species. EE2 accumulation, however, exhibited two distinct patterns, suggesting differences in metabolic processing. In killifish and medaka, the highest accumulation (~ 50%) occurred in the liver and gallbladder, whereas in minnow, goldfish, zebrafish and trout, > 50% accumulated in the carcass. No significant sex differences were found in killifish or minnow, apart from lower gill tissue EE2 accumulation in minnow females. This study demonstrated that metabolic processing of EE2 may be species-specific and tissue specific EE2 distribution profiles vary. These could be indicative of differences in overall EE2 sensitivity among species.
AB - Differences exist among fish species in their sensitivity to endocrine disruptors such as 17-α-ethynylestradiol (EE2). We examined whether there were corresponding differences in EE2 uptake rates and short-term internal distribution patterns. Six freshwater species: Japanese ricefish (medaka, Oryzias latipes), goldfish (Carassius auratus), zebrafish (Danio rerio), fathead minnow (Pimephales promelas), Atlantic killifish (Fundulus heteroclitus) and rainbow trout (Oncorhynchus mykiss) were exposed to waterborne radiolabelled EE2 (100 ng/L) for 2-h measurements of uptake, tissue accumulation and oxygen consumption rates (MO2). EE2 uptake rate and MO2 were relatively consistent among species (2.5-3.0 fold variation), with the only significant differences being a lower EE2 uptake rate in medaka, and lower MO2 in medaka, goldfish, and zebrafish relative to the other species. EE2 accumulation, however, exhibited two distinct patterns, suggesting differences in metabolic processing. In killifish and medaka, the highest accumulation (~ 50%) occurred in the liver and gallbladder, whereas in minnow, goldfish, zebrafish and trout, > 50% accumulated in the carcass. No significant sex differences were found in killifish or minnow, apart from lower gill tissue EE2 accumulation in minnow females. This study demonstrated that metabolic processing of EE2 may be species-specific and tissue specific EE2 distribution profiles vary. These could be indicative of differences in overall EE2 sensitivity among species.
KW - 17α-Ethynylestradiol (EE2)
KW - Fathead minnow
KW - Goldfish
KW - Japanese medaka
KW - Killifish
KW - Rainbow trout
KW - Tissue distribution
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=84893636361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893636361&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2014.01.004
DO - 10.1016/j.cbpc.2014.01.004
M3 - Article
VL - 161
SP - 33
EP - 40
JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
SN - 1532-0456
IS - 1
ER -