A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge

Jeonghyun Ahn, Eun Seok Jun, Hui Sun Lee, Seung Yong Yoon, Dong Hou Kim, Chul Hyun Joo, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-speciflc siRNA is effective against other enteroviruses, siRNAs have potential for a universal antienterovirus strategy.

Original languageEnglish (US)
Pages (from-to)8620-8624
Number of pages5
JournalJournal of Virology
Volume79
Issue number13
DOIs
StatePublished - Jul 1 2005
Externally publishedYes

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Enterovirus
small interfering RNA
HeLa Cells
Small Interfering RNA
cells
virus replication
Antiviral Agents
Coxsackievirus Infections
Sequence Homology
sequence homology
cell death
Cell Death
Down-Regulation
infection

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge. / Ahn, Jeonghyun; Jun, Eun Seok; Lee, Hui Sun; Yoon, Seung Yong; Kim, Dong Hou; Joo, Chul Hyun; Kim, Yoo Kyum; Lee, Heuiran.

In: Journal of Virology, Vol. 79, No. 13, 01.07.2005, p. 8620-8624.

Research output: Contribution to journalArticle

Ahn, Jeonghyun ; Jun, Eun Seok ; Lee, Hui Sun ; Yoon, Seung Yong ; Kim, Dong Hou ; Joo, Chul Hyun ; Kim, Yoo Kyum ; Lee, Heuiran. / A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge. In: Journal of Virology. 2005 ; Vol. 79, No. 13. pp. 8620-8624.
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