A single amino acid change in subunit 6 of the yeast mitochondrial ATPase suppresses a null mutation in ATP10

M. Paul, Antonio Barrientos, A. Tzagoloff

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In an earlier study, the ATP10 gene of Saccharomyces cerevisiae was shown to code for an inner membrane protein required for assembly of the F0 sector of the mitochondrial ATPase complex (Ackerman, S., and Tzagoloff, A. (1990) J. Biol. Chem. 265, 9952-9959). To gain additional insights into the function of Atp10p, we have analyzed a revertant of an atp10 null mutant that displays partial recovery of oligomycin-sensitive ATPase and of respiratory competence. The suppressor mutation in the revertant has been mapped to the OLI2 locus in mitochondrial DNA and shown to be a single base change in the C-terminal coding region of the gene. The mutation results in the substitution of a valine for an alanine at residue 249 of subunit 6 of the ATPase. The ability of the subunit 6 mutation to compensate for the absence of Atp10p implies a functional interaction between the two proteins. Such an interaction is consistent with evidence indicating that the C-terminal region with the site of the mutation and the extramembrane domain of Atp10p are both on the matrix side of the inner membrane. Subunit 6 has been purified from the parental wild type strain, from the atp10 null mutant, and from the revertant. The N-terminal sequences of the three proteins indicated that they all start at Ser11, the normal processing site of the subunit 6 precusor. Mass spectral analysis of the wild type and mutants subunit 6 failed to reveal any substantive difference of the wild type and mutant proteins when the mass of the latter was corrected for Ala → Val mutation. These data argue against a role of Atp10p in post-translational modification of subunit 6. Although post-translational modification of another ATPase subunit interacting with subunit 6 cannot be excluded, a more likely function for Atp10p is that it acts as a subunit 6 chaperone during F0 assembly.

Original languageEnglish
Pages (from-to)29238-29243
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number38
DOIs
StatePublished - Sep 22 2000
Externally publishedYes

Fingerprint

Yeast
Adenosine Triphosphatases
Yeasts
Amino Acids
Mutation
Post Translational Protein Processing
Genes
Valine
Mutant Proteins
Genetic Suppression
Mitochondrial DNA
Alanine
Spectrum analysis
Aptitude
Membrane Proteins
Proteins
Substitution reactions
Mental Competency
Membranes
Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biochemistry

Cite this

A single amino acid change in subunit 6 of the yeast mitochondrial ATPase suppresses a null mutation in ATP10. / Paul, M.; Barrientos, Antonio; Tzagoloff, A.

In: Journal of Biological Chemistry, Vol. 275, No. 38, 22.09.2000, p. 29238-29243.

Research output: Contribution to journalArticle

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