A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart

Boulos Asfour, Joshua Hare, Thomas Kohl, Hideo A. Baba, David A. Kass, Kevin Chen, Tonny D T Tjan, Dieter Hammel, Michael Weyand, Ralph H. Hruban, Hans H. Scheld, Barry J. Byrne

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: The widely used non-volume-loaded abdominal heterotopic heart transplant (NL) in rats undergoes atrophy after transplantation. Various techniques have been designed to load the transplanted heart because of its potential immunological impact. Our aim was to create a volume-loaded heterotopic heart transplantation model (VL) capable of ejection and practical for routine studies. Using this model, we tested the hypothesis that VL isografts would retain myocardial performance comparable to native hearts (NH). Methods: Heterotopic hearts were transplanted using and end-to- side anastomosis between the donor's superior vena cava and the recipient's abdominal inferior vena cava. The right ventricle loads the left ventricle (LV) via a direct anastomosis of the pulmonary artery to the left atrium. The LV ejects volume through an end-to-side anastomosis of the donor's aorta to the recipient's abdominal aorta. Hemodynamic data (systolic and diastolic LV pressures, dP/dt max and min, tau) were studied in-situ (at baseline and after adding volume) and in a Langendorff perfusion system (at baseline and after stimulation with isoproterenol) 2 weeks after transplantation. Results: In situ systolic pressure and diastolic function of VL was superior to NL, and β-adrenergic stimulated performance in the Langendorff perfusion of VL showed hemodynamic performance equivalent to NH, unlike NL which had a diminished response. Conclusion: This technique results in a volume-loaded ejecting heart transplant model that preserves anatomical structures. The VL can be evaluated in situ and after explantation in Langendorff perfusion system and may offer advantages if workload of the graft is of significance to the study performed.

Original languageEnglish
Pages (from-to)927-936
Number of pages10
JournalJournal of Heart and Lung Transplantation
Volume18
Issue number10
DOIs
StatePublished - Oct 1 1999
Externally publishedYes

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Heart Transplantation
Hemodynamics
Heart Ventricles
Perfusion
Transplants
Transplantation
Tissue Donors
Heterotopic Transplantation
Isografts
Anatomic Models
Superior Vena Cava
Abdominal Aorta
Inferior Vena Cava
Workload
Heart Atria
Isoproterenol
Adrenergic Agents
Pulmonary Artery
Atrophy
Aorta

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Transplantation

Cite this

A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart. / Asfour, Boulos; Hare, Joshua; Kohl, Thomas; Baba, Hideo A.; Kass, David A.; Chen, Kevin; Tjan, Tonny D T; Hammel, Dieter; Weyand, Michael; Hruban, Ralph H.; Scheld, Hans H.; Byrne, Barry J.

In: Journal of Heart and Lung Transplantation, Vol. 18, No. 10, 01.10.1999, p. 927-936.

Research output: Contribution to journalArticle

Asfour, Boulos ; Hare, Joshua ; Kohl, Thomas ; Baba, Hideo A. ; Kass, David A. ; Chen, Kevin ; Tjan, Tonny D T ; Hammel, Dieter ; Weyand, Michael ; Hruban, Ralph H. ; Scheld, Hans H. ; Byrne, Barry J. / A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart. In: Journal of Heart and Lung Transplantation. 1999 ; Vol. 18, No. 10. pp. 927-936.
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abstract = "Background: The widely used non-volume-loaded abdominal heterotopic heart transplant (NL) in rats undergoes atrophy after transplantation. Various techniques have been designed to load the transplanted heart because of its potential immunological impact. Our aim was to create a volume-loaded heterotopic heart transplantation model (VL) capable of ejection and practical for routine studies. Using this model, we tested the hypothesis that VL isografts would retain myocardial performance comparable to native hearts (NH). Methods: Heterotopic hearts were transplanted using and end-to- side anastomosis between the donor's superior vena cava and the recipient's abdominal inferior vena cava. The right ventricle loads the left ventricle (LV) via a direct anastomosis of the pulmonary artery to the left atrium. The LV ejects volume through an end-to-side anastomosis of the donor's aorta to the recipient's abdominal aorta. Hemodynamic data (systolic and diastolic LV pressures, dP/dt max and min, tau) were studied in-situ (at baseline and after adding volume) and in a Langendorff perfusion system (at baseline and after stimulation with isoproterenol) 2 weeks after transplantation. Results: In situ systolic pressure and diastolic function of VL was superior to NL, and β-adrenergic stimulated performance in the Langendorff perfusion of VL showed hemodynamic performance equivalent to NH, unlike NL which had a diminished response. Conclusion: This technique results in a volume-loaded ejecting heart transplant model that preserves anatomical structures. The VL can be evaluated in situ and after explantation in Langendorff perfusion system and may offer advantages if workload of the graft is of significance to the study performed.",
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T1 - A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart

AU - Asfour, Boulos

AU - Hare, Joshua

AU - Kohl, Thomas

AU - Baba, Hideo A.

AU - Kass, David A.

AU - Chen, Kevin

AU - Tjan, Tonny D T

AU - Hammel, Dieter

AU - Weyand, Michael

AU - Hruban, Ralph H.

AU - Scheld, Hans H.

AU - Byrne, Barry J.

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Background: The widely used non-volume-loaded abdominal heterotopic heart transplant (NL) in rats undergoes atrophy after transplantation. Various techniques have been designed to load the transplanted heart because of its potential immunological impact. Our aim was to create a volume-loaded heterotopic heart transplantation model (VL) capable of ejection and practical for routine studies. Using this model, we tested the hypothesis that VL isografts would retain myocardial performance comparable to native hearts (NH). Methods: Heterotopic hearts were transplanted using and end-to- side anastomosis between the donor's superior vena cava and the recipient's abdominal inferior vena cava. The right ventricle loads the left ventricle (LV) via a direct anastomosis of the pulmonary artery to the left atrium. The LV ejects volume through an end-to-side anastomosis of the donor's aorta to the recipient's abdominal aorta. Hemodynamic data (systolic and diastolic LV pressures, dP/dt max and min, tau) were studied in-situ (at baseline and after adding volume) and in a Langendorff perfusion system (at baseline and after stimulation with isoproterenol) 2 weeks after transplantation. Results: In situ systolic pressure and diastolic function of VL was superior to NL, and β-adrenergic stimulated performance in the Langendorff perfusion of VL showed hemodynamic performance equivalent to NH, unlike NL which had a diminished response. Conclusion: This technique results in a volume-loaded ejecting heart transplant model that preserves anatomical structures. The VL can be evaluated in situ and after explantation in Langendorff perfusion system and may offer advantages if workload of the graft is of significance to the study performed.

AB - Background: The widely used non-volume-loaded abdominal heterotopic heart transplant (NL) in rats undergoes atrophy after transplantation. Various techniques have been designed to load the transplanted heart because of its potential immunological impact. Our aim was to create a volume-loaded heterotopic heart transplantation model (VL) capable of ejection and practical for routine studies. Using this model, we tested the hypothesis that VL isografts would retain myocardial performance comparable to native hearts (NH). Methods: Heterotopic hearts were transplanted using and end-to- side anastomosis between the donor's superior vena cava and the recipient's abdominal inferior vena cava. The right ventricle loads the left ventricle (LV) via a direct anastomosis of the pulmonary artery to the left atrium. The LV ejects volume through an end-to-side anastomosis of the donor's aorta to the recipient's abdominal aorta. Hemodynamic data (systolic and diastolic LV pressures, dP/dt max and min, tau) were studied in-situ (at baseline and after adding volume) and in a Langendorff perfusion system (at baseline and after stimulation with isoproterenol) 2 weeks after transplantation. Results: In situ systolic pressure and diastolic function of VL was superior to NL, and β-adrenergic stimulated performance in the Langendorff perfusion of VL showed hemodynamic performance equivalent to NH, unlike NL which had a diminished response. Conclusion: This technique results in a volume-loaded ejecting heart transplant model that preserves anatomical structures. The VL can be evaluated in situ and after explantation in Langendorff perfusion system and may offer advantages if workload of the graft is of significance to the study performed.

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