A simple method for estimating association constants between monoclonal antibodies and derivatized or native antigens

W. Schramm, T. Yang, M. E. Meyerhoff, L. G. Bachas

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We describe a simple method for estimating the association constant between an antibody and an antigen, based on a theoretical treatment of experimental dose-response data. We used this method to calculate the association constants of an anti-progesterone monoclonal antibody for native progesterone and for progesterone 11α-hemisuccinate. These association constants provide a quantitative measure of the bridging-group recognition by the antibody.

Original languageEnglish (US)
Pages (from-to)1360-1363
Number of pages4
JournalClinical chemistry
Volume36
Issue number7
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Keywords

  • Antibody characterizaton
  • immunoassay

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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