A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini

P. L. Rutledge, A. Saluja, U. Leli, M. L. Steer

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-fi.m nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.

Original languageEnglish (US)
Pages (from-to)508-511
Number of pages4
JournalPancreas
Volume3
Issue number5
DOIs
StatePublished - Oct 1988
Externally publishedYes

Keywords

  • Enzyme assay
  • Ion fluxes
  • Pancreas
  • Secretion

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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