A simian immunodeficiency virus envelope V3 cytotoxic T-lymphocyte epitope in rhesus monkeys and its restricting major histocompatibility complex class I molecule mamu-A*02

Noriaki Watanabe, Stephen N. McAdam, Jonathan E. Boyson, Marian S. Piekarczyk, Yasuhiro Yasutomi, David I. Watkins, Norman L. Letvin

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34 Scopus citations

Abstract

The use of the simian immunodeficiency virus (SIV) macaque model for assessing human immunodeficiency virus vaccine strategies will be facilitated by the characterization of predominant SIV cytotoxic T-lymphocyte (CTL) epitopes and their restricting major histocompatibility complex (MHC) class I molecules in macaque species. We now define a rhesus monkey SIV(mac) CTL epitope in the third hypervariable region of the envelope glycoprotein of the virus. This epitope, YNLTMKCR, contains the first two amino acids of a cysteine-cysteine loop which is the SIV(mac) analog of the human immunodeficiency virus type 1 V3 loop. We also employed one-dimensional isoelectric focusing to characterize the MHC class I molecule of the rhesus monkey that binds this SIV(mac) envelope peptide fragment. Cloning and sequencing the cDNA encoding this rhesus monkey MHC class I molecule demonstrates that it is a newly described HLA-A homolog, Mamu-A*02. This viral CTL epitope and its restricting MHC class I molecule will facilitate the use of the SIV(mac) rhesus monkey model for studies of envelope-based vaccine strategies and for exploring AIDS immunopathogenesis.

Original languageEnglish (US)
Pages (from-to)6690-6696
Number of pages7
JournalJournal of virology
Volume68
Issue number10
StatePublished - Oct 1 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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