A simian immunodeficiency virus envelope V3 cytotoxic T-lymphocyte epitope in rhesus monkeys and its restricting major histocompatibility complex class I molecule mamu-A*02

Noriaki Watanabe, Stephen N. McAdam, Jonathan E. Boyson, Marian S. Piekarczyk, Yasuhiro Yasutomi, David I. Watkins, Norman L. Letvin

Research output: Contribution to journalArticle

34 Scopus citations


The use of the simian immunodeficiency virus (SIV) macaque model for assessing human immunodeficiency virus vaccine strategies will be facilitated by the characterization of predominant SIV cytotoxic T-lymphocyte (CTL) epitopes and their restricting major histocompatibility complex (MHC) class I molecules in macaque species. We now define a rhesus monkey SIV(mac) CTL epitope in the third hypervariable region of the envelope glycoprotein of the virus. This epitope, YNLTMKCR, contains the first two amino acids of a cysteine-cysteine loop which is the SIV(mac) analog of the human immunodeficiency virus type 1 V3 loop. We also employed one-dimensional isoelectric focusing to characterize the MHC class I molecule of the rhesus monkey that binds this SIV(mac) envelope peptide fragment. Cloning and sequencing the cDNA encoding this rhesus monkey MHC class I molecule demonstrates that it is a newly described HLA-A homolog, Mamu-A*02. This viral CTL epitope and its restricting MHC class I molecule will facilitate the use of the SIV(mac) rhesus monkey model for studies of envelope-based vaccine strategies and for exploring AIDS immunopathogenesis.

Original languageEnglish (US)
Pages (from-to)6690-6696
Number of pages7
JournalJournal of virology
Issue number10
StatePublished - Oct 1 1994
Externally publishedYes


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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