A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria

Nicholas Tsinoremas, Masahiro Ishiura, Takao Kondo, Carol R. Andersson, Kan Tanaka, Hideo Takahashi, Carl H. Johnson, Susan S. Golden

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

We isolated mutants affected in the circadian expression of the psbAI gene in Synechococcus sp. strain PCC 7942 using a strategy that tags the genomic locus responsible for the mutant phenotype. The search identified one short period (22 h) mutant (M2) and two low amplitude mutants, one of which showed apparent arhythmia (M11) and one that was still clearly rhythmic (M16). We characterized the disrupted locus of the low amplitude but still rhythmic mutant (M16) as the rpoD2 gene, a member of a gene family that encodes sigma70-like transcription factors in Synechococcus. We also inactivated rpoD2 in a number of reporter strains and showed that the circadian expression of some genes is not modified by the loss of this sigma factor. Therefore, we conclude that rpoD2 is a component of an output pathway of the biological clock that affects the circadian expression of a subset of genes in Synechococcus. This work demonstrates a direct link between a transcription factor and the manifestation of circadian gene expression.

Original languageEnglish
Pages (from-to)2488-2495
Number of pages8
JournalEMBO Journal
Volume15
Issue number10
StatePublished - May 30 1996
Externally publishedYes

Fingerprint

Synechococcus
Sigma Factor
Cyanobacteria
Genes
Gene Expression
Transcription Factors
Biological Clocks
Phenotype
Gene expression
Clocks

Keywords

  • Circadian rhythm
  • Cyanobacteria
  • psbA
  • Sigma factor
  • Synechococcus

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Tsinoremas, N., Ishiura, M., Kondo, T., Andersson, C. R., Tanaka, K., Takahashi, H., ... Golden, S. S. (1996). A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria. EMBO Journal, 15(10), 2488-2495.

A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria. / Tsinoremas, Nicholas; Ishiura, Masahiro; Kondo, Takao; Andersson, Carol R.; Tanaka, Kan; Takahashi, Hideo; Johnson, Carl H.; Golden, Susan S.

In: EMBO Journal, Vol. 15, No. 10, 30.05.1996, p. 2488-2495.

Research output: Contribution to journalArticle

Tsinoremas, N, Ishiura, M, Kondo, T, Andersson, CR, Tanaka, K, Takahashi, H, Johnson, CH & Golden, SS 1996, 'A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria', EMBO Journal, vol. 15, no. 10, pp. 2488-2495.
Tsinoremas N, Ishiura M, Kondo T, Andersson CR, Tanaka K, Takahashi H et al. A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria. EMBO Journal. 1996 May 30;15(10):2488-2495.
Tsinoremas, Nicholas ; Ishiura, Masahiro ; Kondo, Takao ; Andersson, Carol R. ; Tanaka, Kan ; Takahashi, Hideo ; Johnson, Carl H. ; Golden, Susan S. / A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria. In: EMBO Journal. 1996 ; Vol. 15, No. 10. pp. 2488-2495.
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