A set of compatible tac promoter expression vectors

Derek M. Dykxhoorn, Rebecca St. Pierre, Thomas Linn

Research output: Contribution to journalArticle

158 Scopus citations

Abstract

A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacI(q) gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.

Original languageEnglish (US)
Pages (from-to)133-136
Number of pages4
JournalGene
Volume177
Issue number1-2
DOIs
StatePublished - Oct 24 1996
Externally publishedYes

Keywords

  • β-galactosidase
  • Copy number
  • Gene regulation
  • Incompatibility group
  • LacI gene
  • pUC19 polylinker
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'A set of compatible tac promoter expression vectors'. Together they form a unique fingerprint.

  • Cite this