A set of compatible tac promoter expression vectors

Derek M Dykxhoorn, Rebecca St. Pierre, Thomas Linn

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacI(q) gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.

Original languageEnglish
Pages (from-to)133-136
Number of pages4
JournalGene
Volume177
Issue number1-2
DOIs
StatePublished - Oct 24 1996
Externally publishedYes

Fingerprint

Plasmids
Drug Resistance
Genes
Organism Cloning

Keywords

  • β-galactosidase
  • Copy number
  • Gene regulation
  • Incompatibility group
  • LacI gene
  • pUC19 polylinker
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

A set of compatible tac promoter expression vectors. / Dykxhoorn, Derek M; St. Pierre, Rebecca; Linn, Thomas.

In: Gene, Vol. 177, No. 1-2, 24.10.1996, p. 133-136.

Research output: Contribution to journalArticle

Dykxhoorn, Derek M ; St. Pierre, Rebecca ; Linn, Thomas. / A set of compatible tac promoter expression vectors. In: Gene. 1996 ; Vol. 177, No. 1-2. pp. 133-136.
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