A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs

Richard H. Gomer, Darrell Pilling, Lawrence M. Kauvar, Stote Ellsworth, Sanna D. Ronkainen, David Roife, Stephen C. Davis

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

During wound healing, some circulating monocytes enter the wound, differentiate into fibroblast-like cells called fibrocytes, and appear to then further differentiate into myofibroblasts, cells that play a key role in collagen deposition, cytokine release, and wound contraction. The differentiation of monocytes into fibrocytes is inhibited by the serum protein serum amyloid P (SAP). Depleting SAP at a wound site thus might speed wound healing. SAP binds to some types of agarose in the presence of Ca 2+. We found that human SAP binds to an agarose with a K D of 7 × 10 -8 M and a B max of 2.1 μg SAP/mg wet weight agarose. Mixing this agarose 1 : 5 w/v with 30 μg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca 2+ reduced the free SAP concentration to ∼0.02 μg/mL, well below the concentration that inhibits fibrocyte differentiation. Compared with a hydrogel dressing and a foam dressing, dressings containing this agarose and Ca 2+ significantly increased the speed of wound healing in partial thickness wounds in pigs. This suggests that agarose/Ca 2+ dressings may be beneficial for wound healing in humans.

Original languageEnglish (US)
Pages (from-to)397-404
Number of pages8
JournalWound Repair and Regeneration
Volume17
Issue number3
DOIs
StatePublished - May 2009

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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