A second-generation protein-protein interaction network of helicobacter pylori

Roman Hauser, Arnaud Ceol, Seesandra V. Rajagopala, Roberto Mosca, Gabriella Siszler, Nadja Wermke, Patricia Sikorski, Frank Schwarz, Matthias Schick, Stefan Wuchty, Patrick Aloy, Peter Uetz

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Helicobacter pylori infections cause gastric ulcers and play a major role in the development of gastric cancer. In 2001, the first protein interactome was published for this species, revealing over 1500 binary protein interactions resulting from 261 yeast two-hybrid screens. Here we roughly double the number of previously published interactions using an ORFeome-based, proteome-wide yeast two-hybrid screening strategy. We identified a total of 1515 protein-protein interactions, of which 1461 are new. The integration of all the interactions reported in H. pylori results in 3004 unique interactions that connect about 70% of its proteome. Excluding interactions of promiscuous proteins we derived from our new data a core network consisting of 908 interactions. We compared our data set to several other bacterial interactomes and experimentally benchmarked the conservation of interactions using 365 protein pairs (interologs) of E. coli of which one third turned out to be conserved in both species.

Original languageEnglish (US)
Pages (from-to)1318-1329
Number of pages12
JournalMolecular and Cellular Proteomics
Issue number5
StatePublished - May 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology


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