A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1)

Balakrishna L. Lokeshwar, Hsiu san Lin

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A simple three-layer sandwich enzyme-linked immunoadsorbent assay (sandwich-ELISA) has been developed for murine macrophage colony-stimulating factor-1 (CSF-1) using the two monoclonal antibodies on which we recently reported (J. Immunol. (1988) 141, 483). The anti-CSF-1 monoclonal antibodies used in this assay recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1. This assay is specific to murine CSF-1. Recombinant human macrophage colony-stimulating factor, murine GM-CSF, or IL-3, either alone or together with CSF-1, does not interfere with the assay. The advantage of this assay over other reported immunoassays for CSF-1 is that radiolabeled or large quantities of purified CSF-1 are not required. This sandwich-ELISA compares favorbly with other assays in its rapidity, simplicity, and sensitivity.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalJournal of Immunological Methods
Issue number1
StatePublished - Sep 29 1989



  • Colony-stimulating factor 1
  • In vitro colony assay
  • Monoclonal antibody, rat
  • Sandwich-ELISA

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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