Abstract
A T-lymphoid cell line termed 221 was derived from a rhesus monkey infected with herpesvirus saimiri. Growth of 221 cells was dependent on the addition of interleukin-2 (IL-2) to the culture medium. In the absence of IL- 2, 221 cells arrested in G0-G1 but did not die. Simian immunodeficiency virus (SIV) replicated efficiently in IL-2-stimulated 221 cells whether or not the nef gene was present. In the absence of IL-2, nef-containing SIV replicated 8 to 100 times more efficiently in 221 cells than did the same virus lacking nef. nef-containing virus preferentially stimulated the production of IL-2 from 221 cells. HIV-1 nef and v-ras genes, but not the c- ras gene, were shown to substitute functionally for SIV nef when tested as recombinant viruses in this assay system. These results demonstrate a role for natural nef in causing lymphoid cell activation, and they provide a system for delineating the biochemical mechanisms responsible for this activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6094-6099 |
| Number of pages | 6 |
| Journal | Journal of Virology |
| Volume | 71 |
| Issue number | 8 |
| State | Published - Aug 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
A role for natural simian immunodeficiency virus and human immunodeficiency virus type 1 nef alleles in lymphocyte activation. / Alexander, Louis; Du, Zhenjian; Rosenzweig, Michael; Jung, Jae U.; Desrosiers, Ronald Charles.
In: Journal of Virology, Vol. 71, No. 8, 08.1997, p. 6094-6099.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A role for natural simian immunodeficiency virus and human immunodeficiency virus type 1 nef alleles in lymphocyte activation
AU - Alexander, Louis
AU - Du, Zhenjian
AU - Rosenzweig, Michael
AU - Jung, Jae U.
AU - Desrosiers, Ronald Charles
PY - 1997/8
Y1 - 1997/8
N2 - A T-lymphoid cell line termed 221 was derived from a rhesus monkey infected with herpesvirus saimiri. Growth of 221 cells was dependent on the addition of interleukin-2 (IL-2) to the culture medium. In the absence of IL- 2, 221 cells arrested in G0-G1 but did not die. Simian immunodeficiency virus (SIV) replicated efficiently in IL-2-stimulated 221 cells whether or not the nef gene was present. In the absence of IL-2, nef-containing SIV replicated 8 to 100 times more efficiently in 221 cells than did the same virus lacking nef. nef-containing virus preferentially stimulated the production of IL-2 from 221 cells. HIV-1 nef and v-ras genes, but not the c- ras gene, were shown to substitute functionally for SIV nef when tested as recombinant viruses in this assay system. These results demonstrate a role for natural nef in causing lymphoid cell activation, and they provide a system for delineating the biochemical mechanisms responsible for this activation.
AB - A T-lymphoid cell line termed 221 was derived from a rhesus monkey infected with herpesvirus saimiri. Growth of 221 cells was dependent on the addition of interleukin-2 (IL-2) to the culture medium. In the absence of IL- 2, 221 cells arrested in G0-G1 but did not die. Simian immunodeficiency virus (SIV) replicated efficiently in IL-2-stimulated 221 cells whether or not the nef gene was present. In the absence of IL-2, nef-containing SIV replicated 8 to 100 times more efficiently in 221 cells than did the same virus lacking nef. nef-containing virus preferentially stimulated the production of IL-2 from 221 cells. HIV-1 nef and v-ras genes, but not the c- ras gene, were shown to substitute functionally for SIV nef when tested as recombinant viruses in this assay system. These results demonstrate a role for natural nef in causing lymphoid cell activation, and they provide a system for delineating the biochemical mechanisms responsible for this activation.
UR - http://www.scopus.com/inward/record.url?scp=0030788298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030788298&partnerID=8YFLogxK
M3 - Article
C2 - 9223503
AN - SCOPUS:0030788298
VL - 71
SP - 6094
EP - 6099
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 8
ER -